Elevated corticosteroid receptor expression in thymus of autoimmune-prone (nzbxnzw)fl mice by calorie restriction

V. Durgam, D. Troyer, G. Fernandes

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Glucocorticoids are known to induce apoptosis by binding to liganddependent transcription factors of glucocorticoid receptor sub types. Previous studies have indicated that calorie restriction (CR) accelerates the rate of apoptosis. The present investigation was undertaken to measure the circadian effect on glucocorticoid receptor mRNA expression in thymus of three month old (NZBxNZW)Fl mice fed ad libitum (AL) and calorie-restricted (CR; 40% less calories than AL) diets from one month of age onwards, both in the morning {9 AM) and evening (4 PM). The mRNA levels of both type I (MR) and type II (GR) glucocorticoid receptors were determined by using RT-PCR. Southern blots with the RT-PCR products GR (304 bp) and MR (383 bp) with GA PDH (452 bp) as internal control, were hybridized with cDNA probes labeled with (alpha P-32)dCTP. The intensities of autoradiographed bands were quantified, and the values were expressed as fold change in ratio of the mean densitometric values of GR and MR to that of GAPDH. Our preliminary studies indicate that GR levels are slightly higher in thymus cells of young CR mice (0.76 vs 0.71 for AL). The trend for MR was opposite such that AL animals showed slightly higher expression. In older mice, the same trends were observed. Our findings suggest that increased glucocorticoid receptor expression facilitates increased binding of corticosterone, thereby resulting in elevated apoptosis in CR mice. This may contribute to the retardation of autoimmunity and aging in CR-fed animals. A1392 Study of ImmunotherapY for Respiratory Dise-4e with Low-dosage Inactwated DOG Invaccin Jin-m.ing 2hang. Eric Hu et ni. First Hospital of Hanr-4hou Zhe,1ian P.R.China This series, including clinical oh servit ion 3 . animals experiments pnd nathological examinations, is about th'p therapy for respiratory diseases with low-dosage BCG invncein. All patients nre suffered respectively with asthma, bronchitis, repeated infection in upper-respiratory duct and chronic obstructive pulmonary disease (COPD). Each patient accepta ten items of imm-. unoloffical examinations before and after therapy. Twenty Japan big-ear white rabbit which have been arranged in two groups (ten in control group and ten in therapin.roup) are used to observed the results of the immunological examinations and the pathological findings. The effective rate of 2-42 cases of the children suffered imperatively with asthma is q?£, while the late result r=te qif>. By 52 cases of brochitin, thp effective rate ii 96?-4: 70 cases of reoested infection in upper-respiratory duct, qroi. Those above mentioned children whose inmunolo-4rical indexes are obviously louered. obtain raise of those indexes in different decrees after facing treated with the inactivated BOG for 10 to 20 times (P 0.01). The C0?3 patients, after bein-4 treated for twenty times obtained siKnificard diffprenc with both subpopulations of T cell and lymphocyte transformation rate (LTR) fp 0.01). The animal exoeriment showed thnt, rabits in therapy rroup are significant different fror those in control ffroup by lymphocyte transformation rate (detected with 3H-Thymin):by absolute value of lymphocyte and PHA skin test (P 0.01), Habita in therapy proup have quicker recoueries from totic lesions in heart,liver and kidney by pathological examinations than that in control -4roup. Spleen,appendix .Tid prerrrinal centers in lymnh nodes of the rabits in therany proup are enlarged than that of those in control group. It sugeerts an ?c,iveted proliferation of lymphocytes.

Original languageEnglish (US)
Pages (from-to)A1392
JournalFASEB Journal
Issue number6
StatePublished - Dec 1 1996

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics


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