Electrophoresis of proteins and nucleic acids on acrylamide-agarose gels lacking covalent crosslinking

Paul M. Horowitz, John C. Lee, Geri A. Williams, Robert F. Williams, Larry D. Barnes

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

The preparation of acrylamide-agarose gels lacking covalent crosslinking with methylenebis-acrylamide is described. These hybrid gels melt at 85°C and, consequently, allow quantitative analysis of tritium-labeled protein after electrophoresis. Recovery of tritium-labeled ribonucleic acids extracted from hybrid gels is 20 to 25% greater than from standard acrylamide-methylenebisacrylamide gels. Standard curves of electrophoretic mobilities as a function of molecular weights of dissociated proteins and ribonucleic acids are compared for acrylamide-agarose gels and acrylamide-methylenebisacrylamide gels.

Original languageEnglish (US)
Pages (from-to)333-340
Number of pages8
JournalAnalytical Biochemistry
Volume143
Issue number2
DOIs
StatePublished - Dec 1984

Keywords

  • acrylamide
  • agarose
  • gel electrophoresis
  • nucleic acids
  • proteins

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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