By use of multiple-concentration agarose gels, the distance migrated by linear, duplex DNA during agarose gel electrophoresis has been measured as a function of agarose concentration (A) and molecular weight of the DNA (Mr) for molecules with an Mr between 1.88 × 106 and 110 × 106. Conditions for obtaining a linear plot of log Mr as a function of distance migrated (D) have been found for DNA molecules with an Mr between 5.47 × 106 and 110 × 106; improved conditions for fractionating such molecules are described. The ratio of the D of a DNA with an Mr of 26.5 × 106 to the D of a DNA with an Mr of 77 × 106 was plotted as a function of A and electrical field (E). By use of A values between 0.075 and 0.70% and a constant E, this plot had a single maximum. The maximal value of the above ratio was a decreasing function of E, and the A at this maximal value was also a decreasing function of E.
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