Electron microscopy as a tool for assessment of anticoagulation strategies during extracorporeal life support: the proof is on the membrane

Brendan M. Beely, James E. Campbell, Andrew D Meyer, Thomas Langer, Kathryn Negaard, Kevin K. Chung, Andrew P. Cap, Leopoldo C. Cancio, Andriy I. Batchinsky

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Extracorporeal life support (ECLS) is fast becoming more common place for use in adult patients failing mechanical ventilation. Management of coagulation and thrombosis has long been a major complication in the use of ECLS therapies. Scanning electron microscopy (SEM) of membrane oxygenators (MO) after use in ECLS circuits can offer novel insight into any thrombotic material deposition on the MO. In this pilot study we analyzed five explanted MOs immediately after use in a sheep model of different acute respiratory distress syndrome (ARDS). We describe our methods of MO dissection, sample preparation, image capture, and results. Of the five MOs analyzed, those that received continuous heparin infusion showed very little thrombosis formation or other clot material, while those that were used with only initial heparin bolus showed readily apparent thrombotic material.

Original languageEnglish (US)
JournalASAIO Journal
DOIs
StateAccepted/In press - Jun 1 2016

Fingerprint

Oxygenators
Membrane Oxygenators
Extracorporeal Membrane Oxygenation
Electron microscopy
Electron Microscopy
Membranes
Heparin
Thrombosis
Dissection
Adult Respiratory Distress Syndrome
Coagulation
Artificial Respiration
Electron Scanning Microscopy
Sheep
Scanning electron microscopy
Networks (circuits)
Therapeutics

ASJC Scopus subject areas

  • Biophysics
  • Biomaterials
  • Bioengineering
  • Biomedical Engineering
  • Medicine(all)

Cite this

Electron microscopy as a tool for assessment of anticoagulation strategies during extracorporeal life support : the proof is on the membrane. / Beely, Brendan M.; Campbell, James E.; Meyer, Andrew D; Langer, Thomas; Negaard, Kathryn; Chung, Kevin K.; Cap, Andrew P.; Cancio, Leopoldo C.; Batchinsky, Andriy I.

In: ASAIO Journal, 01.06.2016.

Research output: Contribution to journalArticle

Beely, Brendan M. ; Campbell, James E. ; Meyer, Andrew D ; Langer, Thomas ; Negaard, Kathryn ; Chung, Kevin K. ; Cap, Andrew P. ; Cancio, Leopoldo C. ; Batchinsky, Andriy I. / Electron microscopy as a tool for assessment of anticoagulation strategies during extracorporeal life support : the proof is on the membrane. In: ASAIO Journal. 2016.
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