TY - JOUR
T1 - Eight week exposure to a high sugar high fat diet results in adiposity gain and alterations in metabolic biomarkers in baboons (Papio hamadryas sp.)
AU - Higgins, Paul B.
AU - Bastarrachea, Raul A.
AU - Lopez-Alvarenga, Juan C.
AU - Garcia-Forey, Maggie
AU - Proffitt, J. Michael
AU - Voruganti, V. Saroja
AU - Tejero, M. Elizabeth
AU - Mattern, Vicki
AU - Haack, Karin
AU - Shade, Robert E.
AU - Cole, Shelley A.
AU - Comuzzie, Anthony G.
N1 - Funding Information:
We acknowledge the efforts of Dr. Pat Frost, D.V.M., the Veterinary research technicians, animal caretaker staff, and behavioural staff of the Southwest National Primate Research Centre. We also acknowledge the use of facilities kindly provided by Don Strange of Texas, Inc. This study was funded by the Max and Minnie Tomerlin Voelcker Fund, San Antonio, TX and was conducted using facilities constructed with support from the Research Facilities Improvement Program under grant number C06 RR (numbers 014578, 013556, 015456, 017515) from the National Center for Research Resources and with support from National Institutes of Health grants PO1 HL028972 and P51 RR013986. This work was also supported, in part, by a small equipment grant from the Southwest Foundation for Biomedical Research Founders Council. PBH and JMP were supported by postdoctoral fellowships from the Brackenridge Foundation of San Antonio Texas.
PY - 2010/10/29
Y1 - 2010/10/29
N2 - Background: Baboons (Papio hamadryas Sp.) develop features of the cardiometabolic syndrome and represent a clinically-relevant animal model in which to study the aetiology of the disorder. To further evaluate the baboon as a model for the study of the cardiometabolic syndrome, we developed a high sugar high fat diet and hypothesized that it could be used to induce adiposity gain and affect associated circulating biomarkers.Methods: We developed a diet enriched with monosaccharides and saturated fatty acids that was composed of solid and liquid energy sources. We provided a group of baboons (n = 9) ad libitum access to this diet for 8 weeks. Concurrently, a control group (n = 6) was maintained with ad libitum access to a low sugar low fat baseline diet and normal water for 8 weeks. Body composition was determined by dual-energy X-ray absorptiometry and circulating metabolic biomarkers were measured using standard methodology before and after the 8 week study period.Results: Neither body composition nor circulating biomarkers changed in the control group. Following the 8 weeks, the intervention group had a significant increase in fat mass (1.71 ± 0.98 vs. 3.23 ± 1.70 kg, p = 0.004), triglyceride (55 ± 13 vs. 109 ± 67 mg/dL, p = 0.006,), and leptin (1.19 ± 1.40 vs. 3.29 ± 2.32 ng/mL, p = 0.001) and a decline in adiponectin concentrations (33530 ± 9744 vs. 23330 ± 7863 ng/mL, p = 0.002). Percentage haemoglobin A1C (4.0 ± 0.3 vs. 6.0 ± 1.4, p = 0.002) also increased in the intervention group.Conclusions: Our findings indicate that when exposed to a high sugar high fat diet, young adult male baboons develop increased body fat and triglyceride concentrations, altered adipokine concentrations, and evidence of altered glucose metabolism. Our findings are in keeping with observations in humans and further demonstrate the potential utility of this highly clinically-relevant animal model for studying diet-induced metabolic dysregulation.
AB - Background: Baboons (Papio hamadryas Sp.) develop features of the cardiometabolic syndrome and represent a clinically-relevant animal model in which to study the aetiology of the disorder. To further evaluate the baboon as a model for the study of the cardiometabolic syndrome, we developed a high sugar high fat diet and hypothesized that it could be used to induce adiposity gain and affect associated circulating biomarkers.Methods: We developed a diet enriched with monosaccharides and saturated fatty acids that was composed of solid and liquid energy sources. We provided a group of baboons (n = 9) ad libitum access to this diet for 8 weeks. Concurrently, a control group (n = 6) was maintained with ad libitum access to a low sugar low fat baseline diet and normal water for 8 weeks. Body composition was determined by dual-energy X-ray absorptiometry and circulating metabolic biomarkers were measured using standard methodology before and after the 8 week study period.Results: Neither body composition nor circulating biomarkers changed in the control group. Following the 8 weeks, the intervention group had a significant increase in fat mass (1.71 ± 0.98 vs. 3.23 ± 1.70 kg, p = 0.004), triglyceride (55 ± 13 vs. 109 ± 67 mg/dL, p = 0.006,), and leptin (1.19 ± 1.40 vs. 3.29 ± 2.32 ng/mL, p = 0.001) and a decline in adiponectin concentrations (33530 ± 9744 vs. 23330 ± 7863 ng/mL, p = 0.002). Percentage haemoglobin A1C (4.0 ± 0.3 vs. 6.0 ± 1.4, p = 0.002) also increased in the intervention group.Conclusions: Our findings indicate that when exposed to a high sugar high fat diet, young adult male baboons develop increased body fat and triglyceride concentrations, altered adipokine concentrations, and evidence of altered glucose metabolism. Our findings are in keeping with observations in humans and further demonstrate the potential utility of this highly clinically-relevant animal model for studying diet-induced metabolic dysregulation.
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U2 - 10.1186/1475-2840-9-71
DO - 10.1186/1475-2840-9-71
M3 - Article
C2 - 21034486
AN - SCOPUS:77958570808
VL - 9
JO - Cardiovascular Diabetology
JF - Cardiovascular Diabetology
SN - 1475-2840
M1 - 71
ER -