Abstract
The purpose of this study was to determine calmodulin distribution in platelets of alcoholics. Eight alcoholics were diagnosed by DSM-III (Diagnostic and Statistical Manual of Mental Disorders, 3rd edition) criteria prior to admission to the Alcohol Unit, VA Hospital, San Antonio, TX. Whole blood was drawn after a five day washout period, and again after 30 days of disulfiram treatment. Platelets were prepared from the whole blood, suspended in Ringer's-citrate-dextrose buffer (RCD), divided into 2 aliquots, then sonicated and centrifuged (100K × g). Supernatants were assayed for calmodulin content by RIA. The pellets were resuspended by sonication in 1 mM EGTA in RCD or 1% (w/v) Lubrol-PX in RCD, then centrifuged at 100K × g. Calmodulin was measured in the EGTA and Lubrol supernatants. EGTA-extractable calmodulin in pre-disulfiram, post-disulfiram, and control subjects was 5.07±2.2 (SD), 5.19±1.7 (SD), and 10.5±6.7 (SD) ng calmodulin/mg whole platelet protein. The EGTA-extractable calmodulin was significantly lower in alcoholics pre- or post-disulfiram than in controls (p<0.025). These preliminary results suggest an alteration in platelet membranes of alcoholics that affects the binding of calmodulin.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 433-436 |
| Number of pages | 4 |
| Journal | Alcohol |
| Volume | 2 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1985 |
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Keywords
- Alcoholic
- Calmodulin
- EGTA
- Human
- Membrane
- Platelet
ASJC Scopus subject areas
- Biochemistry
- Medicine(all)
- Neuroscience(all)
- Behavioral Neuroscience
- Toxicology
- Health(social science)
Cite this
EGTA-extractable calmodulin in platelet membrane is lower in alcoholics than in controls. / Javors, Martin A; Houston, J. P.; Bowden, C. L.
In: Alcohol, Vol. 2, No. 3, 1985, p. 433-436.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - EGTA-extractable calmodulin in platelet membrane is lower in alcoholics than in controls
AU - Javors, Martin A
AU - Houston, J. P.
AU - Bowden, C. L.
PY - 1985
Y1 - 1985
N2 - The purpose of this study was to determine calmodulin distribution in platelets of alcoholics. Eight alcoholics were diagnosed by DSM-III (Diagnostic and Statistical Manual of Mental Disorders, 3rd edition) criteria prior to admission to the Alcohol Unit, VA Hospital, San Antonio, TX. Whole blood was drawn after a five day washout period, and again after 30 days of disulfiram treatment. Platelets were prepared from the whole blood, suspended in Ringer's-citrate-dextrose buffer (RCD), divided into 2 aliquots, then sonicated and centrifuged (100K × g). Supernatants were assayed for calmodulin content by RIA. The pellets were resuspended by sonication in 1 mM EGTA in RCD or 1% (w/v) Lubrol-PX in RCD, then centrifuged at 100K × g. Calmodulin was measured in the EGTA and Lubrol supernatants. EGTA-extractable calmodulin in pre-disulfiram, post-disulfiram, and control subjects was 5.07±2.2 (SD), 5.19±1.7 (SD), and 10.5±6.7 (SD) ng calmodulin/mg whole platelet protein. The EGTA-extractable calmodulin was significantly lower in alcoholics pre- or post-disulfiram than in controls (p<0.025). These preliminary results suggest an alteration in platelet membranes of alcoholics that affects the binding of calmodulin.
AB - The purpose of this study was to determine calmodulin distribution in platelets of alcoholics. Eight alcoholics were diagnosed by DSM-III (Diagnostic and Statistical Manual of Mental Disorders, 3rd edition) criteria prior to admission to the Alcohol Unit, VA Hospital, San Antonio, TX. Whole blood was drawn after a five day washout period, and again after 30 days of disulfiram treatment. Platelets were prepared from the whole blood, suspended in Ringer's-citrate-dextrose buffer (RCD), divided into 2 aliquots, then sonicated and centrifuged (100K × g). Supernatants were assayed for calmodulin content by RIA. The pellets were resuspended by sonication in 1 mM EGTA in RCD or 1% (w/v) Lubrol-PX in RCD, then centrifuged at 100K × g. Calmodulin was measured in the EGTA and Lubrol supernatants. EGTA-extractable calmodulin in pre-disulfiram, post-disulfiram, and control subjects was 5.07±2.2 (SD), 5.19±1.7 (SD), and 10.5±6.7 (SD) ng calmodulin/mg whole platelet protein. The EGTA-extractable calmodulin was significantly lower in alcoholics pre- or post-disulfiram than in controls (p<0.025). These preliminary results suggest an alteration in platelet membranes of alcoholics that affects the binding of calmodulin.
KW - Alcoholic
KW - Calmodulin
KW - EGTA
KW - Human
KW - Membrane
KW - Platelet
UR - http://www.scopus.com/inward/record.url?scp=0021969104&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021969104&partnerID=8YFLogxK
U2 - 10.1016/0741-8329(85)90110-7
DO - 10.1016/0741-8329(85)90110-7
M3 - Article
VL - 2
SP - 433
EP - 436
JO - Alcohol
JF - Alcohol
SN - 0741-8329
IS - 3
ER -