EGTA-extractable calmodulin in platelet membrane is lower in alcoholics than in controls

Martin A Javors, J. P. Houston, C. L. Bowden

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The purpose of this study was to determine calmodulin distribution in platelets of alcoholics. Eight alcoholics were diagnosed by DSM-III (Diagnostic and Statistical Manual of Mental Disorders, 3rd edition) criteria prior to admission to the Alcohol Unit, VA Hospital, San Antonio, TX. Whole blood was drawn after a five day washout period, and again after 30 days of disulfiram treatment. Platelets were prepared from the whole blood, suspended in Ringer's-citrate-dextrose buffer (RCD), divided into 2 aliquots, then sonicated and centrifuged (100K × g). Supernatants were assayed for calmodulin content by RIA. The pellets were resuspended by sonication in 1 mM EGTA in RCD or 1% (w/v) Lubrol-PX in RCD, then centrifuged at 100K × g. Calmodulin was measured in the EGTA and Lubrol supernatants. EGTA-extractable calmodulin in pre-disulfiram, post-disulfiram, and control subjects was 5.07±2.2 (SD), 5.19±1.7 (SD), and 10.5±6.7 (SD) ng calmodulin/mg whole platelet protein. The EGTA-extractable calmodulin was significantly lower in alcoholics pre- or post-disulfiram than in controls (p<0.025). These preliminary results suggest an alteration in platelet membranes of alcoholics that affects the binding of calmodulin.

Original languageEnglish (US)
Pages (from-to)433-436
Number of pages4
JournalAlcohol
Volume2
Issue number3
DOIs
StatePublished - 1985

Fingerprint

Egtazic Acid
Calmodulin
Alcoholics
Platelets
alcoholism
Disulfiram
Blood Platelets
Membranes
Citric Acid
Buffers
Glucose
Diagnostic and Statistical Manual of Mental Disorders
mental disorder
edition
Blood
diagnostic
alcohol
Hospital Units
Sonication
Alcohols

Keywords

  • Alcoholic
  • Calmodulin
  • EGTA
  • Human
  • Membrane
  • Platelet

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Neuroscience(all)
  • Behavioral Neuroscience
  • Toxicology
  • Health(social science)

Cite this

EGTA-extractable calmodulin in platelet membrane is lower in alcoholics than in controls. / Javors, Martin A; Houston, J. P.; Bowden, C. L.

In: Alcohol, Vol. 2, No. 3, 1985, p. 433-436.

Research output: Contribution to journalArticle

Javors, Martin A ; Houston, J. P. ; Bowden, C. L. / EGTA-extractable calmodulin in platelet membrane is lower in alcoholics than in controls. In: Alcohol. 1985 ; Vol. 2, No. 3. pp. 433-436.
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