EGFR wild type antagonizes EGFRvIII-mediated activation of Met in glioblastoma

L. Li, V. T. Puliyappadamba, S. Chakraborty, A. Rehman, V. Vemireddy, D. Saha, R. F. Souza, K. J. Hatanpaa, P. Koduru, S. Burma, D. A. Boothman, A. A. Habib

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Epidermal growth factor receptor (EGFR)vIII is the most common EGFR mutant found in glioblastoma (GBM). EGFRvIII does not bind ligand, is highly oncogenic and is usually coexpressed with EGFR wild type (EGFRwt). EGFRvIII activates Met, and Met contributes to EGFRvIII-mediated oncogenicity and resistance to treatment. Here, we report that addition of EGF results in a rapid loss of EGFRvIII-driven Met phosphorylation in glioma cells. Met is associated with EGFRvIII in a physical complex. Addition of EGF results in a dissociation of the EGFRvIII-Met complex with a concomitant loss of Met phosphorylation. Consistent with the abrogation of Met activation, addition of EGF results in the inhibition of EGFRvIII-mediated resistance to chemotherapy. Thus, our study suggests that ligand in the milieu of EGFRvIII-expressing GBM cells is likely to influence the EGFRvIII-Met interaction and resistance to treatment, and highlights a novel antagonistic interaction between EGFRwt and EGFRvIII in glioma cells.

Original languageEnglish (US)
Pages (from-to)129-134
Number of pages6
JournalOncogene
Volume34
Issue number1
DOIs
StatePublished - Jan 2 2015
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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    Li, L., Puliyappadamba, V. T., Chakraborty, S., Rehman, A., Vemireddy, V., Saha, D., Souza, R. F., Hatanpaa, K. J., Koduru, P., Burma, S., Boothman, D. A., & Habib, A. A. (2015). EGFR wild type antagonizes EGFRvIII-mediated activation of Met in glioblastoma. Oncogene, 34(1), 129-134. https://doi.org/10.1038/onc.2013.534