EGF-IL-18 fusion protein as a potential anti-tumor reagent by induction of immune response and apoptosis in cancer cells

Jianxin Lu; Y. Peng; Z. J. Zheng; J. H. Pan; Yangde Zhang; Yidong Bai

doi:10.1016/j.canlet.2007.10.042

EGF-IL-18 fusion protein as a potential anti-tumor reagent by induction of immune response and apoptosis in cancer cells

Jianxin Lu, Y. Peng, Z. J. Zheng, J. H. Pan, Yangde Zhang, Yidong Bai

Department of Cell Systems & Anatomy

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

We report here the generation and characterization of EGF-IL-18 fusion protein as an anti-tumor reagent. The epidermal growth factor (EGF) and interleukin-18 (IL-18) fusion protein was shown to induce interferon-γ (IFNγ) expression and secretion in KG-1 cells, and to promote PBMNC proliferation. It also stimulated activation of CD4⁺ T cells, and increased other immune responses. Moreover, EGF-IL-18 could induce significant tumor regression in SMMC-7721-xenografted Balb/c nude mice when administered together with peritumoral injection of X-ray-irradiated NK-92 cells, and this regression is associated with arresting of the tumor cells in G1 phase and induction of apoptosis.

Original language	English (US)
Pages (from-to)	187-197
Number of pages	11
Journal	Cancer Letters
Volume	260
Issue number	1-2
DOIs	https://doi.org/10.1016/j.canlet.2007.10.042
State	Published - Feb 18 2008

Keywords

Anti-cancer
Apoptosis
Epidermal growth factor (EGF)
Fusion protein
Interleukin-18 (IL-18)

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1016/j.canlet.2007.10.042

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title = "EGF-IL-18 fusion protein as a potential anti-tumor reagent by induction of immune response and apoptosis in cancer cells",

abstract = "We report here the generation and characterization of EGF-IL-18 fusion protein as an anti-tumor reagent. The epidermal growth factor (EGF) and interleukin-18 (IL-18) fusion protein was shown to induce interferon-γ (IFNγ) expression and secretion in KG-1 cells, and to promote PBMNC proliferation. It also stimulated activation of CD4+ T cells, and increased other immune responses. Moreover, EGF-IL-18 could induce significant tumor regression in SMMC-7721-xenografted Balb/c nude mice when administered together with peritumoral injection of X-ray-irradiated NK-92 cells, and this regression is associated with arresting of the tumor cells in G1 phase and induction of apoptosis.",

keywords = "Anti-cancer, Apoptosis, Epidermal growth factor (EGF), Fusion protein, Interleukin-18 (IL-18)",

author = "Jianxin Lu and Y. Peng and Zheng, {Z. J.} and Pan, {J. H.} and Yangde Zhang and Yidong Bai",

note = "Funding Information: This work was supported by the Natural Science Foundation of Zhejiang Province (No. 302769), the Major Program of Zhejiang Medical Science (No. 2001ZD007), the Wenzhou Science and Technology Development Fund Major Project (No. S2001A21) and the Zhejiang Provincial Program for the Cultivation of High-Level Innovative Health talents. Yidong Bai is supported by Windy Will Case Cancer Fund. ",

year = "2008",

month = feb,

day = "18",

doi = "10.1016/j.canlet.2007.10.042",

language = "English (US)",

volume = "260",

pages = "187--197",

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number = "1-2",

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T1 - EGF-IL-18 fusion protein as a potential anti-tumor reagent by induction of immune response and apoptosis in cancer cells

AU - Lu, Jianxin

AU - Peng, Y.

AU - Zheng, Z. J.

AU - Pan, J. H.

AU - Zhang, Yangde

AU - Bai, Yidong

N1 - Funding Information: This work was supported by the Natural Science Foundation of Zhejiang Province (No. 302769), the Major Program of Zhejiang Medical Science (No. 2001ZD007), the Wenzhou Science and Technology Development Fund Major Project (No. S2001A21) and the Zhejiang Provincial Program for the Cultivation of High-Level Innovative Health talents. Yidong Bai is supported by Windy Will Case Cancer Fund.

PY - 2008/2/18

Y1 - 2008/2/18

N2 - We report here the generation and characterization of EGF-IL-18 fusion protein as an anti-tumor reagent. The epidermal growth factor (EGF) and interleukin-18 (IL-18) fusion protein was shown to induce interferon-γ (IFNγ) expression and secretion in KG-1 cells, and to promote PBMNC proliferation. It also stimulated activation of CD4+ T cells, and increased other immune responses. Moreover, EGF-IL-18 could induce significant tumor regression in SMMC-7721-xenografted Balb/c nude mice when administered together with peritumoral injection of X-ray-irradiated NK-92 cells, and this regression is associated with arresting of the tumor cells in G1 phase and induction of apoptosis.

AB - We report here the generation and characterization of EGF-IL-18 fusion protein as an anti-tumor reagent. The epidermal growth factor (EGF) and interleukin-18 (IL-18) fusion protein was shown to induce interferon-γ (IFNγ) expression and secretion in KG-1 cells, and to promote PBMNC proliferation. It also stimulated activation of CD4+ T cells, and increased other immune responses. Moreover, EGF-IL-18 could induce significant tumor regression in SMMC-7721-xenografted Balb/c nude mice when administered together with peritumoral injection of X-ray-irradiated NK-92 cells, and this regression is associated with arresting of the tumor cells in G1 phase and induction of apoptosis.

KW - Anti-cancer

KW - Apoptosis

KW - Epidermal growth factor (EGF)

KW - Fusion protein

KW - Interleukin-18 (IL-18)

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EGF-IL-18 fusion protein as a potential anti-tumor reagent by induction of immune response and apoptosis in cancer cells

Abstract

Keywords

ASJC Scopus subject areas

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