Effects on growth and sporulation of inactivation of a Bacillus subtilis gene (ctc) transcribed in vitro by minor vegetative cell RNA polymerases (E-σ37, E-σ32)

Christine L. Truitt, Elizabeth A. Weaver, William G. Haldenwang

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

The E-σ37 gene ctc was inactivated by a site-specific insertion into the Bacillus subtilis chromosome. The resulting mutation inhibited sporulation by 95% at elevated temperatures (48° C). If the ctc- mutation is placed in a strain that carries a mutation in the closely linked but distinct spoVC gene, ctc now affects both growth and sporulation at elevated temperatures. Growth of the ctc- spoVC285 strain was transiently inhibited when exponentially growing cultures were shifted from 37° C to 48° C. A similar, but less pronounced "growth lag", was also seen in a B. subtilis strain carrying only the spoVC-285 mutation. This finding suggests that both the ctc and spoVC products function in vegetatively growing B. subtilis.

Original languageEnglish (US)
Pages (from-to)166-171
Number of pages6
JournalMGG Molecular & General Genetics
Volume212
Issue number1
DOIs
StatePublished - Apr 1988

Keywords

  • Bacillus
  • RNA polymerase
  • Sporulation

ASJC Scopus subject areas

  • Genetics

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