We studied the effects of vasopressin on phospholipid metabolism in mesangial cells and the temporal relationship of these changes to prostaglandin production. The changes included: 1) increased breakdown of phosphatidylinositol (PI), 2) increased breakdown of diphosphoinositide (PI-P) and triphosphoinositide (PI-P2), 3) an increase in the mass of diglyceride and phosphatidic acid, 4) increased synthesis, first of PI-P and PI-P2, then phosphatidic acid, and, finally, PI, and 5) increased water-soluble inositol phosphates. Vasopressin treatment resulted in a significant increase (90%) in the mass of phosphatidic acid and a smaller (13%) decline in the mass of PI. Changes in diglyceride were seen following 45 s of treatment with vasopressin. Alterations of phosphoinositide metabolism were seen as early as 45 s and continued for up to 5 h following hormone exposure. By contrast, prostaglandin production declined after 30 min. These observations on vasopressin-stimulated metabolism in cultured mesangial cells may provide a basis for an understanding of the functional changes that follow hormone exposure.
|Original language||English (US)|
|Journal||American Journal of Physiology - Renal Fluid and Electrolyte Physiology|
|State||Published - 1985|
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