Effects of suramin on human platelet aggregation and Ca2+ mobilization induced by thrombin and other agonists

Athanassia Siafaka-Kapadai, Stanislaus Svetlov, Donald J. Hanahan, Martin A. Javors

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The purpose of this study was to investigate the effect of suramin, a polyanionic napthalene sulfonic acid, on human platelet aggregation and Ca2+ mobilization induced by various agonists. Our results show that suramin completely inhibited aggregation by thrombin, platelet activating factor (PAF), alkyllysophosphatidic acid (ALPA), or arachidonic acid in a concentration-dependent manner. The IC50 values of suramin for inhibition of aggregation by PAF, arachidonic acid, and thrombin were 76.7, 239, and 1.49 μg/ml, respectively. Ca2+ mobilization induced by thrombin was inhibited by suramin with an approximate IC50 value of 20 μg/ml. This concentration of suramin had no effect on PAF or oleic acid-induced Ca2+ mobilization. The mechanism by which suramin inhibits aggregation is not clear, but our results suggest that suramin inhibits the ligand-receptor interaction.

Original languageEnglish (US)
Pages (from-to)1769-1777
Number of pages9
JournalLife Sciences
Volume63
Issue number20
DOIs
StatePublished - Oct 9 1998

Keywords

  • ALPA
  • Arachidonic acid
  • Ca mobilization
  • PAF
  • Platelet aggregation
  • Suramin
  • Thrombin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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