Effects of reconstituted HDL on charge-based LDL subfractions as characterized by capillary isotachophoresis

Bo Zhang, Yoshinari Uehara, Satoru Hida, Shin Ichiro Miura, David L. Rainwater, Masaru Segawa, Koichiro Kumagai, Kerry Anne Rye, Keijiro Saku

    Research output: Contribution to journalArticle

    10 Scopus citations

    Abstract

    Modified LDL in human plasma including small, dense LDL (sdLDL) and oxidized LDL carries a more negative charge than unmodified LDL and is atherogenic. We examined the effects of apolipoprotein A-I (apoA-I)/POPC discs on charge-based LDL subfractions as determined by capillary isotachophoresis (cITP). Three normal healthy subjects and seven patients with metabolic disorders were included in the study. LDL in human plasma was separated into two major subfractions, fast- and slow-migrating LDL (fLDL and sLDL), by cITP. Normal LDL was characterized by low fLDL, and mildly oxidized LDL in vitro and mildly modified LDL in human plasma were characterized by increased fLDL. Moderately oxidized LDL in vitro and moderately modified LDL in a patient with hypertriglyceridemia and HDL deficiency were characterized by both increased fLDL and a new LDL subfraction with a faster mobility than fLDL [very-fast-migrating LDL as determined by cITP (vfLDL)]. cITP LDL subfractions with faster electrophoretic mobility (fLDL vs. sLDL, vfLDL vs. fLDL) were associated with an increased content of sdLDL. Incubation of a plasma fraction with d > 1.019 g/ml (depleted of triglyceride-rich lipoproteins) in the presence of apoA-I/POPC discs at 37°C greatly decreased vfLDL and fLDL but increased sLDL. Incubation of whole plasma from patients with an altered distribution of cITP LDL subfractions in the presence of apoA-I/ POPC discs also greatly decreased fLDL but increased sLDL. ApoA-I/POPC discs decreased the cITP fLDL level, the free cholesterol concentration, and platelet-activating factor acetylhydrolase activity in the sdLDL subclasses (d = 1.040-1.063 g/ml) and increased the size of LDL. ApoA-I/POPC discs reduced charge-modified LDL in human plasma by remodeling cITP fLDL into sLDL subfractions.

    Original languageEnglish (US)
    Pages (from-to)1175-1189
    Number of pages15
    JournalJournal of lipid research
    Volume48
    Issue number5
    DOIs
    StatePublished - May 2007

    Keywords

    • Charge-based low density lipoprotein subfractions
    • Low density lipoprotein size
    • Plasma density > 1.019 g/ml fraction
    • Small, dense low density lipoprotein subclasses

    ASJC Scopus subject areas

    • Biochemistry
    • Endocrinology
    • Cell Biology

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  • Cite this

    Zhang, B., Uehara, Y., Hida, S., Miura, S. I., Rainwater, D. L., Segawa, M., Kumagai, K., Rye, K. A., & Saku, K. (2007). Effects of reconstituted HDL on charge-based LDL subfractions as characterized by capillary isotachophoresis. Journal of lipid research, 48(5), 1175-1189. https://doi.org/10.1194/jlr.M600227-JLR200