Effects of radiation on Ca2+ signaling in salivary epithelial cell lines transfected with Bcl-2 and Bcl-XL

Xiuhua Sun, Xiao Bing Liu, J. Ricardo Martinez, Howard Dang, Guo H. Zhang

Research output: Contribution to journalArticle

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Abstract

The effects of radiation on the Ca2+ signaling system in HSY cells transfected with the Bcl-2 or Bel-XL gene were studied. Bcl-2 overexpression did not alter carbachol (CCh)-elicited initial increase in cytosolic free Ca2+ concentrations ([Ca2+]i), but Bel-XL overexpression dramatically reduced this response. Exposure to 10 Gy γ-ray did not alter basal [Ca2+]i. By contrast, the CCh-stimulated initial [Ca2+]i increase was reduced at 0.5 and 4 h post-irradiation in all cell types and remained decreased at 24 h in wild-type and control-transfected cells, but recovered in Bcl-2- and Bcl-XL-transfectants. The formation of inositol 1,4,5-trisphosphate (IP3) in response to CCh at 4-h post-irradiation was decreased in wild-type and control-transfected cells, but not in Bcl-2 and Bel-XL transfectants. The capacity of the IP3-sensitive Ca2+ store was significantly reduced by radiation in all cells except Bel-XL transfectants. Ca2+ influx after stimulation with CCh was suppressed by exposure to radiation in wild-type and control-transfected cells, but not in Bcl-2- and Bcl-XL-transfectants. However, radiation enhanced Ca2+ influx activated by thapsigargin in all cell types. These results suggest that 1) radiation diminishes IP3 formation and Ca2+ release in response to CCh, but potentiates the store-operated Ca2+ influx; and 2) overexpression of Bcl-2 or Bel-XL partially protects cells from radiation-induced inhibition of Ca2+ signaling.

Original languageEnglish (US)
Pages (from-to)103-108
Number of pages6
JournalEuropean Journal of Oral Sciences
Volume109
Issue number2
StatePublished - Apr 2001

Fingerprint

Radiation Effects
Epithelial Cells
Carbachol
Cell Line
Radiation
Inositol 1,4,5-Trisphosphate
Thapsigargin
Genes

Keywords

  • Bcl-2
  • Bcl-XL
  • Ca release and influx
  • HSY cells
  • IP formation

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Effects of radiation on Ca2+ signaling in salivary epithelial cell lines transfected with Bcl-2 and Bcl-XL. / Sun, Xiuhua; Liu, Xiao Bing; Martinez, J. Ricardo; Dang, Howard; Zhang, Guo H.

In: European Journal of Oral Sciences, Vol. 109, No. 2, 04.2001, p. 103-108.

Research output: Contribution to journalArticle

Sun, Xiuhua ; Liu, Xiao Bing ; Martinez, J. Ricardo ; Dang, Howard ; Zhang, Guo H. / Effects of radiation on Ca2+ signaling in salivary epithelial cell lines transfected with Bcl-2 and Bcl-XL. In: European Journal of Oral Sciences. 2001 ; Vol. 109, No. 2. pp. 103-108.
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