Effects of preload and eicosanoid synthesis inhibition on rat aortic smooth muscle sensitivity

E. Porsa, M. J.K. Harper, J. T. Herlihy

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Concentration-response curves to serotonin and phenylephrine were obtained from aortic strips subjected to low (0.75 g) and high (3.0) preloads in the presence and absence of eicosanoid synthesis inhibitors. The sensitivity of the strips to both agonists was greater in the high preload strips. The cyclooxygenase inhibitor, indomethacin (28 μM), shifted the serotonin concentration-response curves to the right. However, the preload effect still remained. The lipoxygenase inhibitor, nordihydroguaiaretic acid (10μM), not only decreased sensitivity to serotonin and phenylephrine, but eliminated the preload effect as well. These results suggest that 1) both cyclooxygenase and lipoxygenase metabolites affect the sensitivity of isolated arterial smooth muscle to vasoactive agents, and 2) lipoxygenase, but not cyclooxygenase, metabolites may play a role in the effect of preload on arterial smooth muscle sensitivity.

Original languageEnglish (US)
Pages (from-to)61-65
Number of pages5
JournalProstaglandins, Leukotrienes and Essential Fatty Acids
Issue number1
StatePublished - Oct 1989

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology


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