Effects of peptidoglycans from periodontal pathogens on selected biological activities of CD-1 murine peritoneal macrophages

M. Barnard, S. C. Holt

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Resident CD-1 murine peritoneal macrophages were exposed to various concentrations of purified peptidoglycan isolated from members of the genera Bacteroides, Eikenella, and Actinomyces. Macrophage viability, the release of lysozyme, acid phosphatase, and prostaglandins E1 and E2 were assayed as a function of peptidoglycan concentration and time. Macrophages responded as a function of peptidoglycan concentration with increased release of acid phosphatase and prostaglandins; all cells remained >90% viable during the course of the experiments. However, concentrations of peptidoglycan >50 μg/mL were toxic to the macrophages, while the peptidoglycan from B. capillus strain 925.08 and Actinomyces viscosus strain T14AV consumed complement by both the classical and the alternate pathways. Cellular lysozyme activity and phagocytosis of Saccharomyces cerevisiae were significantly reduced in the presence of peptidoglycan. When viewed by scanning electron microscopy, the activated macrophages were rounded, lacked distinct pseudopod extensions, and possessed an increased number of microvilli and plasma membrane associated vesicles. These morphological alterations occurred as early as 3 h. Transmission electron microscopy revealed the purified peptidoglycan to have been taken up into numerous phagosomes; however, even after 24 h incubation, it was only partially degraded.

Original languageEnglish (US)
Pages (from-to)161-172
Number of pages12
JournalCanadian Journal of Microbiology
Volume31
Issue number2
DOIs
StatePublished - Jan 1 1985

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Effects of peptidoglycans from periodontal pathogens on selected biological activities of CD-1 murine peritoneal macrophages'. Together they form a unique fingerprint.

Cite this