Effects of nitric oxide scavenging on sheep pancreas and liver during sepsis

H. Bone, P. Schenarts, L. Cindrick, L. Traber, D. Traber

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


We investigated the effects of the nitric oxide scavenger pyridoxalated hemoglobin polyoxyethylene conjugate (PHP) on regional blood flow (Qr) and enzyme activity in pancreas and liver during sepsis. Sheep (n=14) received an infusion of live Ps. aeruginosa bacteria for 48 hours. After 24 hours of sepsis the animals were randomly assigned to either a control (CON, n=7, saline), or a treatment group (PHP, n=7, 100mg HP/kg over 30 min). Qr (in ml/min/g) to pancreas (Qpanc) and liver (Qtiver) were measured with colored microsphercs, and serum enzyme activity (in U/L) of lipase, aspartate amino transferase (AST), and alanine amino transferase (ALT) was determined. There was no increase in liver or pancreas enzyme activity, and there was no significant decrease in organ perfusion after PHP infusion. Lipase AST ALT Qnanc Qliver baseline CON I85±28 87± 8.0 1 31±1.4 3.3±0.5 0.029±0.005 PHP220±68 133±18 39±5.3 2.6±0.2 0.057±0.019 24 h CON 189±43 110±6.4 32±2.0 3.0±0.6 0.10±0.022 PHP 150±34 133±14 37±4.7 2.9±0.3 0.23±0.094 25 h CON 180±41 110±6.5 31±2.0 2.5±0.2 0.14±0.028 PHP 122±29 138±13 30± 4.5 1 1.9±0.2 0.18±.047 =p<.05 vs baseline. 1= P<.05 vs. 24 h. 2 =p<.05 vs PHP.

Original languageEnglish (US)
Pages (from-to)A598
JournalFASEB Journal
Issue number3
StatePublished - 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics


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