Effects of maternal nutrient restriction, intrauterine growth restriction, and glucocorticoid exposure on phosphoenolpyruvate carboxykinase-1 expression in fetal baboon hepatocytes in vitro

Cun Li, Zhen Ju Shu, Shuko Lee, Madhulika B. Gupta, Thomas Jansson, Peter W. Nathanielsz, Amrita Kamat

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Background: The objective of this study was to develop a cell culture system for fetal baboon hepatocytes and to test the hypotheses that (i) expression of the gluconeogenic enzyme phosphoenolpyruvate carboxykinase-1 (PEPCK-1) is upregulated in hepatocytes isolated from fetuses of nutrient-restricted mothers (MNR) compared with ad libitum-fed controls (CTR), and (ii) glucocorticoids stimulate PEPCK-1 expression. Methods: Hepatocytes from 0.9G CTR and MNR fetuses were isolated and cultured. PEPCK-1 protein and mRNA levels in hepatocytes were determined by Western blot and quantitative PCR, respectively. Results: Fetuses of MNR mothers were intrauterine growth restricted (IUGR). Feasibility of culturing 0.9G fetal baboon hepatocytes was demonstrated. PEPCK-1 protein levels were increased in hepatocytes isolated from IUGR fetuses, and PEPCK-1 mRNA expression was stimulated by glucocorticoids in fetal hepatocytes. Conclusions: Cultured fetal baboon hepatocytes that retain their in vivo phenotype provide powerful in vitro tools to investigate mechanisms that regulate normal and programmed hepatic function.

Original languageEnglish (US)
Pages (from-to)211-219
Number of pages9
JournalJournal of medical primatology
Volume42
Issue number4
DOIs
StatePublished - Aug 2013

Keywords

  • Dexamethasone
  • Diabetes non-human primate
  • Liver

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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