Effects of intravenous dimethyl sulfoxide on ischemia evolution in a rat permanent occlusion model

Juergen Bardutzky, Xianjun Meng, James Bouley, Timothy Q. Duong, Rajiv Ratan, Marc Fisher

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Dimethyl sulfoxide (DMSO) has a variety of biological actions that suggest efficacy as a neuroprotectant. We (1) tested the neuroprotective potential of DMSO at different time windows on infarct size using 2,3,5-triphenyltetrazolium staining and (2) investigated the effects of DMSO on ischemia evolution using quantitative diffusion and perfusion imaging in a permanent middle cerebral artery occlusion (MCAO) model in rats. In experiment 1, DMSO treatment (1.5 g/kg intravenously over 3h) reduced infarct volume 24 h after MCAO by 65% (P < 0.00001) when initiated 20 h before MCAO, by 44% (P = 0.0006) when initiated 1 h after MCAO, and by 17% (P = 0.11) when started 2 h after MCAO. Significant infarct reduction was also observed after a 3-day survival in animals treated 1 h after MCAO (P = 0.005). In experiment 2, treatment was initiated 1 h after MCAO and maps for cerebral blood flow (CBF) and apparent diffusion coefficient (ADC) were acquired before treatment and then every 30 mins up to 4 h. Cerebral blood flow characteristics and CBF-derived lesion volumes did not differ between treated and untreated animals, whereas the ADC-derived lesion volume essentially stopped progressing during DMSO treatment, resulting in a persistent diffusion/perfusion mismatch. This effect was mainly observed in the cortex. Our data suggest that DMSO represents an interesting candidate for acute stroke treatment.

Original languageEnglish (US)
Pages (from-to)968-977
Number of pages10
JournalJournal of Cerebral Blood Flow and Metabolism
Volume25
Issue number8
DOIs
StatePublished - Aug 2005

Keywords

  • Diffusion/perfusion mismatch
  • Dimethyl sulfoxide
  • Focal brain ischemia
  • Neuroprotection

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

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