Effects of exercise and l-arginine on ventricular remodeling and oxidative stress

Xiaohua Xu, Weiyan Zhao, Shunhua Lao, Bryan S. Wilson, John M Erikson, John Q. Zhang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

OBJECTIVE: Our aim was to characterize the changes in messenger RNA (mRNA) abundance, protein, and activity levels of the enzymatic antioxidants, superoxide dismutase (SOD), glutathione peroxidase, and catalase by exercise training combined with l-arginine after myocardial infarction (MI). Methods: l-Arginine (1 g•kg•d) and N-nitro-l-arginine methyl ester (l-NAME; 10 mg•kg•d) were administered in drinking water for 8 wk. Sprague-Dawley rats were randomized to the following groups: sham-operated control (Sham); MI sedentary (Sed); MI exercise (Ex); MI sedentary + l-arginine (Sed + LA); MI exercise + l-arginine (Ex + LA); MI sedentary + l-NAME (Sed + l-NAME); and MI exercise + l-NAME (Ex + l-NAME). Results: The glutathione peroxidase, catalase, and gp91 mRNA levels were comparable among all the groups. The SOD mRNA level was significantly increased in the Ex group (5.43 ± 0.87) compared with the Sed group (1.74 ± 0.29), whereas this effect was pronouncedly down-regulated by the l-NAME intervention (2.51 ± 1.17, P < 0.05). The protein levels of SOD in the Sed and Ex groups were both significantly decreased with the administration of l-NAME. The protein levels of catalase were significantly higher in the Ex and Ex + LA groups than that in the Sed, Sed + LA, and l-NAME-treated groups. The collagen volume fraction was significantly lowered by the exercise and/or l-arginine treatment when compared with the Sed group. Fractional shortening was significantly preserved in the trained groups compared with their corresponding sedentary groups with or without drug treatments. However, the beneficial effect was not further improved by l-arginine treatment. Conclusions: Our results suggest that exercise training exerts antioxidative effects and attenuates myocardial fibrosis in the MI rats. These improvements, in turn, alleviate cardiac stiffness and preserve post-MI cardiac function. In addition, l-arginine appears to have no additive effect on cardiac function or expression of enzymatic antioxidants.

Original languageEnglish (US)
Pages (from-to)346-354
Number of pages9
JournalMedicine and Science in Sports and Exercise
Volume42
Issue number2
DOIs
StatePublished - Feb 2010

Fingerprint

Ventricular Remodeling
Arginine
Oxidative Stress
Myocardial Infarction
Catalase
Superoxide Dismutase
Glutathione Peroxidase
Messenger RNA
Antioxidants
Exercise
Proteins
Drinking Water
Sprague Dawley Rats
Fibrosis
Collagen

Keywords

  • Cardiac function
  • Exercise training
  • Free radicals
  • Myocardial infarction

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Effects of exercise and l-arginine on ventricular remodeling and oxidative stress. / Xu, Xiaohua; Zhao, Weiyan; Lao, Shunhua; Wilson, Bryan S.; Erikson, John M; Zhang, John Q.

In: Medicine and Science in Sports and Exercise, Vol. 42, No. 2, 02.2010, p. 346-354.

Research output: Contribution to journalArticle

Xu, Xiaohua ; Zhao, Weiyan ; Lao, Shunhua ; Wilson, Bryan S. ; Erikson, John M ; Zhang, John Q. / Effects of exercise and l-arginine on ventricular remodeling and oxidative stress. In: Medicine and Science in Sports and Exercise. 2010 ; Vol. 42, No. 2. pp. 346-354.
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T1 - Effects of exercise and l-arginine on ventricular remodeling and oxidative stress

AU - Xu, Xiaohua

AU - Zhao, Weiyan

AU - Lao, Shunhua

AU - Wilson, Bryan S.

AU - Erikson, John M

AU - Zhang, John Q.

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N2 - OBJECTIVE: Our aim was to characterize the changes in messenger RNA (mRNA) abundance, protein, and activity levels of the enzymatic antioxidants, superoxide dismutase (SOD), glutathione peroxidase, and catalase by exercise training combined with l-arginine after myocardial infarction (MI). Methods: l-Arginine (1 g•kg•d) and N-nitro-l-arginine methyl ester (l-NAME; 10 mg•kg•d) were administered in drinking water for 8 wk. Sprague-Dawley rats were randomized to the following groups: sham-operated control (Sham); MI sedentary (Sed); MI exercise (Ex); MI sedentary + l-arginine (Sed + LA); MI exercise + l-arginine (Ex + LA); MI sedentary + l-NAME (Sed + l-NAME); and MI exercise + l-NAME (Ex + l-NAME). Results: The glutathione peroxidase, catalase, and gp91 mRNA levels were comparable among all the groups. The SOD mRNA level was significantly increased in the Ex group (5.43 ± 0.87) compared with the Sed group (1.74 ± 0.29), whereas this effect was pronouncedly down-regulated by the l-NAME intervention (2.51 ± 1.17, P < 0.05). The protein levels of SOD in the Sed and Ex groups were both significantly decreased with the administration of l-NAME. The protein levels of catalase were significantly higher in the Ex and Ex + LA groups than that in the Sed, Sed + LA, and l-NAME-treated groups. The collagen volume fraction was significantly lowered by the exercise and/or l-arginine treatment when compared with the Sed group. Fractional shortening was significantly preserved in the trained groups compared with their corresponding sedentary groups with or without drug treatments. However, the beneficial effect was not further improved by l-arginine treatment. Conclusions: Our results suggest that exercise training exerts antioxidative effects and attenuates myocardial fibrosis in the MI rats. These improvements, in turn, alleviate cardiac stiffness and preserve post-MI cardiac function. In addition, l-arginine appears to have no additive effect on cardiac function or expression of enzymatic antioxidants.

AB - OBJECTIVE: Our aim was to characterize the changes in messenger RNA (mRNA) abundance, protein, and activity levels of the enzymatic antioxidants, superoxide dismutase (SOD), glutathione peroxidase, and catalase by exercise training combined with l-arginine after myocardial infarction (MI). Methods: l-Arginine (1 g•kg•d) and N-nitro-l-arginine methyl ester (l-NAME; 10 mg•kg•d) were administered in drinking water for 8 wk. Sprague-Dawley rats were randomized to the following groups: sham-operated control (Sham); MI sedentary (Sed); MI exercise (Ex); MI sedentary + l-arginine (Sed + LA); MI exercise + l-arginine (Ex + LA); MI sedentary + l-NAME (Sed + l-NAME); and MI exercise + l-NAME (Ex + l-NAME). Results: The glutathione peroxidase, catalase, and gp91 mRNA levels were comparable among all the groups. The SOD mRNA level was significantly increased in the Ex group (5.43 ± 0.87) compared with the Sed group (1.74 ± 0.29), whereas this effect was pronouncedly down-regulated by the l-NAME intervention (2.51 ± 1.17, P < 0.05). The protein levels of SOD in the Sed and Ex groups were both significantly decreased with the administration of l-NAME. The protein levels of catalase were significantly higher in the Ex and Ex + LA groups than that in the Sed, Sed + LA, and l-NAME-treated groups. The collagen volume fraction was significantly lowered by the exercise and/or l-arginine treatment when compared with the Sed group. Fractional shortening was significantly preserved in the trained groups compared with their corresponding sedentary groups with or without drug treatments. However, the beneficial effect was not further improved by l-arginine treatment. Conclusions: Our results suggest that exercise training exerts antioxidative effects and attenuates myocardial fibrosis in the MI rats. These improvements, in turn, alleviate cardiac stiffness and preserve post-MI cardiac function. In addition, l-arginine appears to have no additive effect on cardiac function or expression of enzymatic antioxidants.

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