Effects of dietary wheat bran fiber on rectal epithelial cell proliferation in patients with research for colorectal cancers

D. S. Alberts, J. Einspahr, S. Rees-mcgee, P. Ramanujam, M. K. Buller, L. Clark, C. Ritenbaugh, J. Atwood, P. Pethigal, D. Earnest, H. Villar, J. Phelps, M. Lipkin, M. Wargovich, F. L. Meyskens

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Abstract

A preponderance of carcinogenesis studies in rodents and epidemiologic studies in humans suggests a potential role of dietary fiber in the prevention of colorectal cancer. Recently, wheat bran fiber used as a dietary supplement has been shown to decrease the growth of rectal adenomatous polyps in patients with familial poluposis; however, few studies of high-risk human populatins have been attempted to determine the effects of dietary fiber supplementation on markersn of carcinogenesis in the colon or rectum. We have designed a one-arm study to evaluate the effects of dietary supplementation with wheat bran fiber [i.e., 13.5 g/day for 8 wk; after 1 mo, 2 g/day (compliance evaluation period)] on [3H]thy-midine rectal mucosa cell labeling (i.e., percent of epithelial cells incorporating [3H]thymidine into DNA in intact rectal crypt cells over a 90-min exposure as well as in minced rectal biopsy tissue over a 24-hr exposure) in rectal biopsy speciamens. The biopsy specimens were obtained at sigmoidoscopy in 17 compliant patients with a history of resected colon or rectal cancer. We categorized patients as having initaially low or initially high [3H]thymidine-labeling indices (i.e., percent of mucosa cells that incorporate [3H]thymidine into DNA during 1.5-or 24-hour in vitro incubations) by using the median baseline labeling index as a cutoff between high and low values. On the basis of a chi-square test used to identify patients with a statistically significant (P<. 001) change, six of the eight patients who initially had high 24-hour outgrowth labeling indices showed a significant decrease in the rectal mucosa biopsy specimens obtained after treatment. An over-all 22% decrease was observed in rectal mucosa cell biopsy specimens obtained at study termination (P<.001). Of the eight patients with initially high total [3H]thymidine-labeling indices in crypt organ culture, four had a significant (P <.001) decrease from baseline values, one had a significant increase, and three showed no change following the fiber intervention. The wheat bran fiber dietary supplement of 13.5 g/day was well tolerated by this group of older (54-70 yr) patients. Although the [3H]-thymidine labeling index data suggest that the wheat bran fiber supplement can inhibit DNA synthesis and rectal mucosa cell proliferation in high-risk patients, the resulta of this small pilot study should not be overinterpreted vis á vis the potential role of wheat bran fiber as a chemopreventive agent for colorectal cancer. Study results should be confirmed in the setting of a randomized double-blined clinical trial in colorectal cancer patients. Intermediated markers of carcinogenesis should be used as end points. [J Natl Cancer Inst 82: 1280-1285, 1990]

Original languageEnglish (US)
Pages (from-to)1280-1285
Number of pages6
JournalJournal of the National Cancer Institute
Volume82
Issue number15
DOIs
StatePublished - Aug 1 1990

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Alberts, D. S., Einspahr, J., Rees-mcgee, S., Ramanujam, P., Buller, M. K., Clark, L., Ritenbaugh, C., Atwood, J., Pethigal, P., Earnest, D., Villar, H., Phelps, J., Lipkin, M., Wargovich, M., & Meyskens, F. L. (1990). Effects of dietary wheat bran fiber on rectal epithelial cell proliferation in patients with research for colorectal cancers. Journal of the National Cancer Institute, 82(15), 1280-1285. https://doi.org/10.1093/jnci/82.15.1280