Effects of dietary polyunsaturated and saturated fats on lipoproteins in the baboon

John Babiak, Alex V. Nichols, Elaine L. Gong, C. Alex McMahan, Thomas J. Kuehl, Glen E. Mott, Henry C. McGill

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The effects of 2 different dietary fats (40% of calories from corn oil or coconut oil), in the presence of high-dietary cholesterol (1.7 mg/kcal), on the lipoprotein profiles of baboons (Papio cynocephalus sp) were studied by analytic ultracentrifugation, gradient gel electrophoresis (GGE), and heparin-manganese chloride precipitation. Relative to the corn oil (polyunsaturated fat) diet, the coconut oil (saturated fat) diet significantly increased total serum cholesterol by 43% (P < 0.001) by increasing non-precipitable cholesterol (HDL-C) 58% (P < 0.001) and precipitable cholesterol (VLDL + LDL-C) 35% of (P < 0.001). Analytic ultracentrifugal observations indicated that the increase in HDL-C was due to considerable increases in both HDL-I (baboon HDL of size 100-125 Å and hydrated density 1.063-1.120 g/ml) and F1.200 9-28 lipoproteins (material of size 125-220 Å and hydrated density 1.03-1.08 g/ml, and containing HDL apolipoproteins and apo E). Concentrations of other HDL subpopulations were unaffected by the dietary saturated rat. The increase in VLDL + LDL-C was due to increased LDL (SF0 5-12), and to some extent, F of the F1.200 9-28 lipoproteins were precipitable by heparin-manganese. In contrast, saturated fat (relative to polyunsaturated fat) induced lower concentrations of IDL saturated fat (relative to polyunsaturated fat) induced lower concentrations of IDL (SF0 12-20) and VLDL (SF0 20-100). Lipoprotein size distributions by GGE indicated 5 HDL subpopulations and 2 or more LDL subpopulations in the sera of most baboons. The type of dietary fat did not affect the particle size range of each of the the HDL or LDL subpopulations. The results indicate that dietary fat markedly modulates the distribution of cholesterol between apo A-I-containing (HDL and F1.200 9-28) and apo B-containing (IDL and VLDL) lipoproteins without altering the presence of subpopulations based on particle size.

Original languageEnglish (US)
Pages (from-to)1-17
Number of pages17
JournalAtherosclerosis
Volume57
Issue number1
DOIs
Publication statusPublished - Oct 1985

    Fingerprint

Keywords

  • Analytic ultracentrifugation
  • Baboons
  • Cholesterol feeding
  • F 9-28 lipoproteins
  • Heparin
  • Polyunsaturated fats
  • Saturated fats
  • manganese chloride precipitation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Babiak, J., Nichols, A. V., Gong, E. L., McMahan, C. A., Kuehl, T. J., Mott, G. E., & McGill, H. C. (1985). Effects of dietary polyunsaturated and saturated fats on lipoproteins in the baboon. Atherosclerosis, 57(1), 1-17. https://doi.org/10.1016/0021-9150(85)90133-9