Effects of dietary N-6 and N-3 lipid consumption and calorie restriction on intestinal immune function in old B/W mice

B. O. Lim, F. Ernani, B. P. Yu, G. Fernandes

Research output: Contribution to journalArticle


Sources of dietary lipids and calorie restriction are known to modulate immune function of splenic T cells. The present study was carried out to compare the intestinal immune system in NZBxNZW (B/W) female mice fed either ad libitum or calorie-restricted diets containing 10% (w/w) corn oil (n-6) or fish oil (n-3). One-month-old mice were divided into four groups: group 1, corn oil fed ad libitum (COAL); group 2, corn oil fed, calorie-restricted (COCR); group 3, ash oil fed ad libitum (FOAL); group 4, fish oil, calorie-restricted (FOCR). Mice (8 months old) were sacrificed and mesenteric lymph nodes (MLN) were collected and cultured to measure the in vitro immune response. Results show MLN lymphocytes from COCR mice produced significantly less IgA, IgG1, IgG2a and IgG3 when compared to cells from COAL fed mice. Also, MLN from COCR and FOCR mice secreted markedly reduced levels of the cytokine IL-10 and were followed by an increased percentage of CD4+ T cells and decreased Ig+ cells. Further, serum IgM and IgG3 concentrations in FOCR mice were significantly (p < 0.05) reduced when compared to COAL and FOAL mice. Also, serum IgE concentrations in COCR and FOCR mice were found significantly lower man that in COAL and FOAL. In summary, these studies suggest that calorie restriction significantly modulates MLN lymphocyte subsets and their function involved in the intestinal immune system.

Original languageEnglish (US)
Pages (from-to)A870
JournalFASEB Journal
Issue number5
StatePublished - Mar 20 1998


ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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