Abstract
Background: Oxidative stress plays a major role in disease processes such as atherosclerosis and diabetes. Peroxynitrite is a reaction product of nitric oxide (NO) and superoxide and a potent oxidant. The peroxynitrite-mediated tyrosine nitration, which forms nitrotyrosine (NT), is associated with several pathological conditions. Methods: We measured plasma NT levels using the HPLC method in 40 Mexican Americans with diabetes, but not taking medications, and 40 age- and sex-matched euglycaemic controls. Results: Plasma-free NT levels were not different between subjects with diabetes (11.0 ± 1.7 nmol/l, n = 40) and with non-diabetes (10.4 ± 1.5 nmol/l, n = 40). There was also no association with levels of fasting glucose (r = -0.049, P = 0.663) or 2-h glucose (r = -0.099, P = 0.390). However, females had significantly lower free NT level (7.6 ± 1.4 nmol/l, n = 40) than males (13.8 ± 1.7 nmol/l, n = 40, P = 0.005), which were not affected by age, smoking status, BMI and glucose levels. Conclusions: In contrast to some earlier reports, our study shows that diabetes has no effect on plasma NT levels in Mexican Americans. We have also demonstrated lower free NT levels in females than males, which may partly explain the lower risk profile to vascular disease in women.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 577-580 |
| Number of pages | 4 |
| Journal | Diabetic Medicine |
| Volume | 21 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 2004 |
| Externally published | Yes |
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Keywords
- Diabetes
- Hyperglycaemia
- Nitrosative stress
- Nitrotyrosine
- Oxidative stress
ASJC Scopus subject areas
- Endocrinology
- Internal Medicine
- Endocrinology, Diabetes and Metabolism
Cite this
Effects of diabetes on plasma nitrotyrosine levels. / Wang, X. L.; Rainwater, D. L.; Leone, A.; Mahaney, M. C.
In: Diabetic Medicine, Vol. 21, No. 6, 06.2004, p. 577-580.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of diabetes on plasma nitrotyrosine levels
AU - Wang, X. L.
AU - Rainwater, D. L.
AU - Leone, A.
AU - Mahaney, M. C.
PY - 2004/6
Y1 - 2004/6
N2 - Background: Oxidative stress plays a major role in disease processes such as atherosclerosis and diabetes. Peroxynitrite is a reaction product of nitric oxide (NO) and superoxide and a potent oxidant. The peroxynitrite-mediated tyrosine nitration, which forms nitrotyrosine (NT), is associated with several pathological conditions. Methods: We measured plasma NT levels using the HPLC method in 40 Mexican Americans with diabetes, but not taking medications, and 40 age- and sex-matched euglycaemic controls. Results: Plasma-free NT levels were not different between subjects with diabetes (11.0 ± 1.7 nmol/l, n = 40) and with non-diabetes (10.4 ± 1.5 nmol/l, n = 40). There was also no association with levels of fasting glucose (r = -0.049, P = 0.663) or 2-h glucose (r = -0.099, P = 0.390). However, females had significantly lower free NT level (7.6 ± 1.4 nmol/l, n = 40) than males (13.8 ± 1.7 nmol/l, n = 40, P = 0.005), which were not affected by age, smoking status, BMI and glucose levels. Conclusions: In contrast to some earlier reports, our study shows that diabetes has no effect on plasma NT levels in Mexican Americans. We have also demonstrated lower free NT levels in females than males, which may partly explain the lower risk profile to vascular disease in women.
AB - Background: Oxidative stress plays a major role in disease processes such as atherosclerosis and diabetes. Peroxynitrite is a reaction product of nitric oxide (NO) and superoxide and a potent oxidant. The peroxynitrite-mediated tyrosine nitration, which forms nitrotyrosine (NT), is associated with several pathological conditions. Methods: We measured plasma NT levels using the HPLC method in 40 Mexican Americans with diabetes, but not taking medications, and 40 age- and sex-matched euglycaemic controls. Results: Plasma-free NT levels were not different between subjects with diabetes (11.0 ± 1.7 nmol/l, n = 40) and with non-diabetes (10.4 ± 1.5 nmol/l, n = 40). There was also no association with levels of fasting glucose (r = -0.049, P = 0.663) or 2-h glucose (r = -0.099, P = 0.390). However, females had significantly lower free NT level (7.6 ± 1.4 nmol/l, n = 40) than males (13.8 ± 1.7 nmol/l, n = 40, P = 0.005), which were not affected by age, smoking status, BMI and glucose levels. Conclusions: In contrast to some earlier reports, our study shows that diabetes has no effect on plasma NT levels in Mexican Americans. We have also demonstrated lower free NT levels in females than males, which may partly explain the lower risk profile to vascular disease in women.
KW - Diabetes
KW - Hyperglycaemia
KW - Nitrosative stress
KW - Nitrotyrosine
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=2942600997&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2942600997&partnerID=8YFLogxK
U2 - 10.1111/j.1464-5491.2004.01211.x
DO - 10.1111/j.1464-5491.2004.01211.x
M3 - Article
VL - 21
SP - 577
EP - 580
JO - Diabetic Medicine
JF - Diabetic Medicine
SN - 0742-3071
IS - 6
ER -