Effects of diabetes on plasma nitrotyrosine levels

X. L. Wang, D. L. Rainwater, A. Leone, M. C. Mahaney

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: Oxidative stress plays a major role in disease processes such as atherosclerosis and diabetes. Peroxynitrite is a reaction product of nitric oxide (NO) and superoxide and a potent oxidant. The peroxynitrite-mediated tyrosine nitration, which forms nitrotyrosine (NT), is associated with several pathological conditions. Methods: We measured plasma NT levels using the HPLC method in 40 Mexican Americans with diabetes, but not taking medications, and 40 age- and sex-matched euglycaemic controls. Results: Plasma-free NT levels were not different between subjects with diabetes (11.0 ± 1.7 nmol/l, n = 40) and with non-diabetes (10.4 ± 1.5 nmol/l, n = 40). There was also no association with levels of fasting glucose (r = -0.049, P = 0.663) or 2-h glucose (r = -0.099, P = 0.390). However, females had significantly lower free NT level (7.6 ± 1.4 nmol/l, n = 40) than males (13.8 ± 1.7 nmol/l, n = 40, P = 0.005), which were not affected by age, smoking status, BMI and glucose levels. Conclusions: In contrast to some earlier reports, our study shows that diabetes has no effect on plasma NT levels in Mexican Americans. We have also demonstrated lower free NT levels in females than males, which may partly explain the lower risk profile to vascular disease in women.

Original languageEnglish (US)
Pages (from-to)577-580
Number of pages4
JournalDiabetic Medicine
Volume21
Issue number6
DOIs
StatePublished - Jun 2004
Externally publishedYes

Fingerprint

Peroxynitrous Acid
Glucose
Vascular Diseases
Oxidants
Superoxides
Tyrosine
3-nitrotyrosine
Fasting
Atherosclerosis
Nitric Oxide
Oxidative Stress
Smoking
High Pressure Liquid Chromatography

Keywords

  • Diabetes
  • Hyperglycaemia
  • Nitrosative stress
  • Nitrotyrosine
  • Oxidative stress

ASJC Scopus subject areas

  • Endocrinology
  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Wang, X. L., Rainwater, D. L., Leone, A., & Mahaney, M. C. (2004). Effects of diabetes on plasma nitrotyrosine levels. Diabetic Medicine, 21(6), 577-580. https://doi.org/10.1111/j.1464-5491.2004.01211.x

Effects of diabetes on plasma nitrotyrosine levels. / Wang, X. L.; Rainwater, D. L.; Leone, A.; Mahaney, M. C.

In: Diabetic Medicine, Vol. 21, No. 6, 06.2004, p. 577-580.

Research output: Contribution to journalArticle

Wang, XL, Rainwater, DL, Leone, A & Mahaney, MC 2004, 'Effects of diabetes on plasma nitrotyrosine levels', Diabetic Medicine, vol. 21, no. 6, pp. 577-580. https://doi.org/10.1111/j.1464-5491.2004.01211.x
Wang, X. L. ; Rainwater, D. L. ; Leone, A. ; Mahaney, M. C. / Effects of diabetes on plasma nitrotyrosine levels. In: Diabetic Medicine. 2004 ; Vol. 21, No. 6. pp. 577-580.
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