Effects of cartilage-derived morphogenetic protein-3 on the expression of chondrogenic and osteoblastic markers in the pluripotent mesenchymal C3H10T1/2 cell line

Lee Chuan C. Yeh, John C. Lee

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

CDMP-3/GDF-7/BMP-12 treatment of pluripotent mesenchymal C3H10T1/2 cells resulted in a dose- and time-dependent change in cell morphology and in the expression of alkaline phosphatase, mRNA expression of osteocalcin, and bone sialoprotein, as well as mineralized bone nodule formation. CDMP-3 also stimulated Alcian Blue staining indicative of extracellular matrix formation without affecting aggrecan expression. CDMP-3 downregulated mRNA expression of BMP-4 and BMP-8A. CDMP-3 stimulated mRNA expression of ALK-1, ALK-2(ActR-IA), ALK-3(BMPR-IA), and ALK-4 without affecting that of ALK-6(BMPRIB), ALK-7, and BMPR-II. These findings suggest that, under the experimental conditions studied, CDMP-3 induces the pluripotent mesenchymal C3H10T1/2 cells to express both chondrocytic and osteoblastic markers. The results further reveal potential complex interplay between the different bone morphogenetic proteins and their receptors in these processes.

Original languageEnglish (US)
Pages (from-to)117-128
Number of pages12
JournalGrowth Factors
Volume28
Issue number2
DOIs
StatePublished - Apr 1 2010

Keywords

  • Bone morphogenetic protein-12
  • C3H10T1/2 cells
  • Cartilage-derived morphogenetic protein-3
  • Chondrogenesis
  • Growth and differentiation factor-7
  • Osteoblastogenesis

ASJC Scopus subject areas

  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

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