Effects of age and caloric restriction on lipid peroxidation: Measurement of oxidative stress by F2-isoprostane levels

Walter F. Ward, Wenbo Qi, Holly Van Remmen, William E. Zackert, L. Jackson Roberts, Arlan Richardson

Research output: Contribution to journalArticlepeer-review

94 Scopus citations


The free radical theory of aging proposes that the accumulation of oxidative damage is a key component of the aging process. The discovery of F2-isoprostanes (F2-isoPs) and their establishment as a sensitive and accurate biomarker of lipid peroxidation represents a major advance for measuring the oxidative stress status of an organism. We have shown that plasma free and total (free plus esterified) F2-isoPs increase with age (185% and 66%, respectively), and that these increases are reduced by life-extending caloric restriction (50% and 23%, respectively). In addition, we found that levels of esterified F2-isoPs increase 68% with age in liver, and 76% with age in kidney. Caloric restriction modulated the age-related increase, reducing the esterified F2-isoPs levels 27% in liver and 35% in kidney. These age-related increases in esterified F2-isoPs levels correlate well with DNA oxidation, as measured by 8-oxodeoxyguanosine production demonstrating that F2-isoPs are an excellent biomarker for age-related changes in oxidative damage to membranes.

Original languageEnglish (US)
Pages (from-to)847-851
Number of pages5
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Issue number7
StatePublished - Jul 2005

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology


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