TY - JOUR
T1 - Effects of age and caloric restriction on lipid peroxidation
T2 - Measurement of oxidative stress by F2-isoprostane levels
AU - Ward, Walter F.
AU - Qi, Wenbo
AU - Van Remmen, Holly
AU - Zackert, William E.
AU - Roberts, L. Jackson
AU - Richardson, Arlan
N1 - Funding Information:
ACKNOWLEDGMENTS This work was supported by a Merit Review grant (HVR, AR), an Environmental Hazards Center grant from the Department of Veteran Affairs (HVR, AR), and by National Institutes of Health grants R01 AG025362-01 (WW), R37 GM42056 (Merit Award to LJR), and R01 AG23843 (AR).
PY - 2005/7
Y1 - 2005/7
N2 - The free radical theory of aging proposes that the accumulation of oxidative damage is a key component of the aging process. The discovery of F2-isoprostanes (F2-isoPs) and their establishment as a sensitive and accurate biomarker of lipid peroxidation represents a major advance for measuring the oxidative stress status of an organism. We have shown that plasma free and total (free plus esterified) F2-isoPs increase with age (185% and 66%, respectively), and that these increases are reduced by life-extending caloric restriction (50% and 23%, respectively). In addition, we found that levels of esterified F2-isoPs increase 68% with age in liver, and 76% with age in kidney. Caloric restriction modulated the age-related increase, reducing the esterified F2-isoPs levels 27% in liver and 35% in kidney. These age-related increases in esterified F2-isoPs levels correlate well with DNA oxidation, as measured by 8-oxodeoxyguanosine production demonstrating that F2-isoPs are an excellent biomarker for age-related changes in oxidative damage to membranes.
AB - The free radical theory of aging proposes that the accumulation of oxidative damage is a key component of the aging process. The discovery of F2-isoprostanes (F2-isoPs) and their establishment as a sensitive and accurate biomarker of lipid peroxidation represents a major advance for measuring the oxidative stress status of an organism. We have shown that plasma free and total (free plus esterified) F2-isoPs increase with age (185% and 66%, respectively), and that these increases are reduced by life-extending caloric restriction (50% and 23%, respectively). In addition, we found that levels of esterified F2-isoPs increase 68% with age in liver, and 76% with age in kidney. Caloric restriction modulated the age-related increase, reducing the esterified F2-isoPs levels 27% in liver and 35% in kidney. These age-related increases in esterified F2-isoPs levels correlate well with DNA oxidation, as measured by 8-oxodeoxyguanosine production demonstrating that F2-isoPs are an excellent biomarker for age-related changes in oxidative damage to membranes.
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U2 - 10.1093/gerona/60.7.847
DO - 10.1093/gerona/60.7.847
M3 - Article
C2 - 16079206
AN - SCOPUS:23244442108
VL - 60
SP - 847
EP - 851
JO - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
JF - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
SN - 1079-5006
IS - 7
ER -