Effects of 1α(OH)-vitamin D3 and 24,25(OH)2-vitamin D3 on long bones of glucocorticoid-treated rats

J. Turnquist, A. Ornoy, D. Eini, Zvi Schwartz

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Glucocorticoids may induce osteopenia in experimental animals and in man. In order to study the possible effects of vitamin D metabolites in the prevention of glucocorticoid-induced osteopenia in rats, we administered 1α(OH)-vitamin D3, 24,25(OH)2-vitamin D3 or a combination of both metabolites, by intragastric intubation, to rats treated daily by intramuscular injections of 10 mg/kg cortisone acetate. Treatment with the vitamin D metabolites started after 1 month of glucocorticoid therapy, at the time osteopenia was already present. Cortisone acetate decreased the gain weight, increased alkaline phosphatase (AP) and decreased Ca serum levels. It also decreased tibial wet and ash weight and tibial Ca content. Computerized histomorphometry of sections from the upper tibia showed decreased epiphyseal bone volume and increased bone marrow volume; decreased height of hypertrophic cartilage in the growth plate and decreased amount of persisting cartilage in the metaphyseal bone trabeculae were also observed. Administration of 24,25(OH)2D3 alone did not reduce these glucocorticoid-induced bone changes and sometimes even worsened them. 1α(OH)D3 reversed many of the deleterious effects of cortisone acetate. It reduced serum AP levels, increased serum Ca levels, increased bone ash weight, epiphyseal and metaphyseal bone volume, with a concomitant reduction in epiphyseal and metaphyseal bone marrow volume. The best results were obtained by a combination of 1α(OH)D3 and 24,25(OH)2D3. It is presumed that both metabolites are needed to reduce the impact of glucocorticoids on bone. 1α(OH)2D3 acts on the gut, increasing Ca absorption (which was decreased by glucocorticoids), and 24,25(OH)2D3 directly acts on bone to enhance bone formation and mineralization.

Original languageEnglish (US)
Pages (from-to)61-67
Number of pages7
JournalActa Anatomica
Volume145
Issue number1
StatePublished - 1992
Externally publishedYes

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Cholecalciferol
Glucocorticoids
Bone and Bones
Metabolic Bone Diseases
Vitamin D
Cartilage
Alkaline Phosphatase
Bone Marrow
Serum
Weights and Measures
Physiologic Calcification
Growth Plate
Intramuscular Injections
Tibia
Intubation
Osteogenesis
Weight Gain
Therapeutics
cortisone acetate

ASJC Scopus subject areas

  • Anatomy

Cite this

Turnquist, J., Ornoy, A., Eini, D., & Schwartz, Z. (1992). Effects of 1α(OH)-vitamin D3 and 24,25(OH)2-vitamin D3 on long bones of glucocorticoid-treated rats. Acta Anatomica, 145(1), 61-67.

Effects of 1α(OH)-vitamin D3 and 24,25(OH)2-vitamin D3 on long bones of glucocorticoid-treated rats. / Turnquist, J.; Ornoy, A.; Eini, D.; Schwartz, Zvi.

In: Acta Anatomica, Vol. 145, No. 1, 1992, p. 61-67.

Research output: Contribution to journalArticle

Turnquist, J, Ornoy, A, Eini, D & Schwartz, Z 1992, 'Effects of 1α(OH)-vitamin D3 and 24,25(OH)2-vitamin D3 on long bones of glucocorticoid-treated rats', Acta Anatomica, vol. 145, no. 1, pp. 61-67.
Turnquist, J. ; Ornoy, A. ; Eini, D. ; Schwartz, Zvi. / Effects of 1α(OH)-vitamin D3 and 24,25(OH)2-vitamin D3 on long bones of glucocorticoid-treated rats. In: Acta Anatomica. 1992 ; Vol. 145, No. 1. pp. 61-67.
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