Effects of β-adrenergic blockade on hepatic and renal glucose production during hypoglycemia in conscious dogs

Eugenio Cersosimo, Irina N. Zaitseva, Mohamed Ajmal

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

To investigate the role of β-adrenergic mechanisms in the counterregulatory response of the liver and kidney to hypoglycemia, we studied 10 dogs before and after a 2-h constant infusion of insulin (4 mU · kg-1 · min-1) either without (n = 4) or with (8 μg/min, n = 6) propranolol and variable dextrose to maintain hypoglycemia, 7 days after surgical placement of sampling catheters in left renal and hepatic veins and femoral artery. Systemic glucose appearance (R(a)) and endogenous (EGP), hepatic (HGP), and renal (RGP) glucose production were measured by a combination of arteriovenous difference and peripheral infusion of [6- 3H]glucose, renal blood flow with a flow probe, and hepatic plasma flow by indocyanine green clearance. Without β-adrenergic blockade, arterial glucose decreased from 5.12 ± 0.02 to 2.53 ± 0.07 mmol/l, glucose R(a) increased from 17.8 ± 0.7 to 30.5 ± 2.5 (P < 0.01) when EGP was 22.2 ± 0.5, HGP from 13.5 ± 1.1 to 19.3 ± 1.3, and RGP from 2.4 ± 1.0 to 8.6 ± 0.9 μmol · kg-1 · min-1 (all P < 0.05). When propranolol was infused, glucose decreased from 5.97 ± 0.02 to 2.71 ± 0.03 mmol/l, glucose R(a) increased from 16.3 ± 1.0 to 25.1 ± 1.6 when EGP was 9.9 ± 0.4, HGP decreased from 14.4 ± 0.7 to 10.4 ± 0.6, and RGP decreased from 3.8 ± 1.3 to 1.1 ± 0.8 μmol · kg-1 · min-1 (all P < 0.05). Our data indicate that β- adrenergic blockade impairs glucose recovery during sustained hypoglycemia, in part, by preventing the simultaneous compensatory increase in HGP and RGP.

Original languageEnglish (US)
Pages (from-to)E792-E797
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume275
Issue number5 38-5
DOIs
StatePublished - 1998

Keywords

  • Carbohydrate
  • Counterregulation
  • Kidney
  • Liver
  • Propranolol

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

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