Effect of volunteer wheel exercise on lymphocyte subsets in ad libitum and food restricted C57BL/6 mice

M. Yoshida, W. Zhao, R. M. McCarter, G. Fernandes

Research output: Contribution to journalArticlepeer-review


Moderate exercise is recommended to maintain health and immunity optimally. Food restriction (40%) in rodents is regularly found to increase lifespan by decreasing body weight. The present volunteer wheel exercise study monitored with digital meters was undertaken in 4 mo old C57BL/6 female mice maintained singly on diets given ad libitum (AL) or 40% calorie restriction (CR) with or without access to running wheel for 4 weeks. Weekly body weights and daily food intake and running speed were recorded closely. After 4 weeks, both sedentary (Sed) and exercised (Ex) mice were bled retroorbitally under anesthesia to collect 100 μl blood and CD4+, CD8+ and CD19+ cells were determined by FACScan. The results showed that AL fed Ex mice showed 20% decreased weight whereas CR Ex mice showed no significant difference. FACS analysis showed 0.38 (CD4:CD8) ratio for AL Sed, 0.39 for AL Ex, 0.41 for CR Sed and 0.44 for FR Ex. It appears CR+Ex increases CD4/CD8 ratio more than AL+Ex. Also, lymphocyte subset and intracellular cytokine production is followed monthly in the blood throughout exercise period without sacrificing the animals. Although AL and CR fed mice exercised equally in the first and 2nd week (4-5 miles/day), CR mice however began to run less during 3rd and 4th week. In summary our results indicate mouse peripheral blood can be utilized sequentially to measure changes in lymphocyte subsets during volunteer exercise.

Original languageEnglish (US)
Pages (from-to)A53
JournalFASEB Journal
Issue number3
StatePublished - 1997

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics


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