Abstract
A progressive decline in the specific binding of [20-3H]phorbol 12,13-dibutyrate ([3H]PDBu) and glycoprotein synthesis was observed following treatment of primary mouse epidermal cells with tunicamycin, a specific inhibitor of dolichol-mediated glycosylation. Following 18 h of treatment, the specific binding of [3H]PDBu was reduced to 33-56% of the control value. The total protein synthesis determined by leucine incorporation into acid-insoluble material was not altered by this antibiotic drug. These results suggest that the receptor for phorbol diesters is, or is functionally linked to, a glycoprotein on the cell surface.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 319-325 |
| Number of pages | 7 |
| Journal | Cancer Letters |
| Volume | 16 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1982 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cancer Research
- Molecular Biology
- Oncology
Cite this
Effect of tunicamycin on receptors for tumor promoters. / Solanki, V.; Logani, M. K.; Slaga, Thomas J.
In: Cancer Letters, Vol. 16, No. 3, 1982, p. 319-325.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of tunicamycin on receptors for tumor promoters
AU - Solanki, V.
AU - Logani, M. K.
AU - Slaga, Thomas J
PY - 1982
Y1 - 1982
N2 - A progressive decline in the specific binding of [20-3H]phorbol 12,13-dibutyrate ([3H]PDBu) and glycoprotein synthesis was observed following treatment of primary mouse epidermal cells with tunicamycin, a specific inhibitor of dolichol-mediated glycosylation. Following 18 h of treatment, the specific binding of [3H]PDBu was reduced to 33-56% of the control value. The total protein synthesis determined by leucine incorporation into acid-insoluble material was not altered by this antibiotic drug. These results suggest that the receptor for phorbol diesters is, or is functionally linked to, a glycoprotein on the cell surface.
AB - A progressive decline in the specific binding of [20-3H]phorbol 12,13-dibutyrate ([3H]PDBu) and glycoprotein synthesis was observed following treatment of primary mouse epidermal cells with tunicamycin, a specific inhibitor of dolichol-mediated glycosylation. Following 18 h of treatment, the specific binding of [3H]PDBu was reduced to 33-56% of the control value. The total protein synthesis determined by leucine incorporation into acid-insoluble material was not altered by this antibiotic drug. These results suggest that the receptor for phorbol diesters is, or is functionally linked to, a glycoprotein on the cell surface.
UR - http://www.scopus.com/inward/record.url?scp=0019959080&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019959080&partnerID=8YFLogxK
U2 - 10.1016/0304-3835(82)90013-1
DO - 10.1016/0304-3835(82)90013-1
M3 - Article
C2 - 6295623
AN - SCOPUS:0019959080
VL - 16
SP - 319
EP - 325
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 3
ER -