Effect of tunicamycin on receptors for tumor promoters

V. Solanki; M. K. Logani; T. J. Slaga

doi:10.1016/0304-3835(82)90013-1

Effect of tunicamycin on receptors for tumor promoters

V. Solanki, M. K. Logani, T. J. Slaga

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Abstract

A progressive decline in the specific binding of [20-³H]phorbol 12,13-dibutyrate ([³H]PDBu) and glycoprotein synthesis was observed following treatment of primary mouse epidermal cells with tunicamycin, a specific inhibitor of dolichol-mediated glycosylation. Following 18 h of treatment, the specific binding of [³H]PDBu was reduced to 33-56% of the control value. The total protein synthesis determined by leucine incorporation into acid-insoluble material was not altered by this antibiotic drug. These results suggest that the receptor for phorbol diesters is, or is functionally linked to, a glycoprotein on the cell surface.

Original language	English (US)
Pages (from-to)	319-325
Number of pages	7
Journal	Cancer Letters
Volume	16
Issue number	3
DOIs	https://doi.org/10.1016/0304-3835(82)90013-1
State	Published - Sep 1982
Externally published	Yes

ASJC Scopus subject areas

Oncology
Cancer Research

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@article{b42f015267264d799e3080095c6ace31,

title = "Effect of tunicamycin on receptors for tumor promoters",

abstract = "A progressive decline in the specific binding of [20-3H]phorbol 12,13-dibutyrate ([3H]PDBu) and glycoprotein synthesis was observed following treatment of primary mouse epidermal cells with tunicamycin, a specific inhibitor of dolichol-mediated glycosylation. Following 18 h of treatment, the specific binding of [3H]PDBu was reduced to 33-56% of the control value. The total protein synthesis determined by leucine incorporation into acid-insoluble material was not altered by this antibiotic drug. These results suggest that the receptor for phorbol diesters is, or is functionally linked to, a glycoprotein on the cell surface.",

author = "V. Solanki and Logani, {M. K.} and Slaga, {T. J.}",

note = "Funding Information: This researchw as sponsored by the Office of Health and Environmental Research, U.S. Department of Energy, under contract W-7405-eng-26w ith the Union Carbide Corporation. VS. is a postdoctoral Investigator supported by Subcontract No. 3322 from the Biology Division of Oak Ridge National Laboratory to the University of Tennessee.M .K.L. is on study leave from the Department of Dermatology, Temple University Health Sciences Center, Philadelphia, PA 19140.",

year = "1982",

month = sep,

doi = "10.1016/0304-3835(82)90013-1",

language = "English (US)",

volume = "16",

pages = "319--325",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "3",

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TY - JOUR

T1 - Effect of tunicamycin on receptors for tumor promoters

AU - Solanki, V.

AU - Logani, M. K.

AU - Slaga, T. J.

N1 - Funding Information: This researchw as sponsored by the Office of Health and Environmental Research, U.S. Department of Energy, under contract W-7405-eng-26w ith the Union Carbide Corporation. VS. is a postdoctoral Investigator supported by Subcontract No. 3322 from the Biology Division of Oak Ridge National Laboratory to the University of Tennessee.M .K.L. is on study leave from the Department of Dermatology, Temple University Health Sciences Center, Philadelphia, PA 19140.

PY - 1982/9

Y1 - 1982/9

N2 - A progressive decline in the specific binding of [20-3H]phorbol 12,13-dibutyrate ([3H]PDBu) and glycoprotein synthesis was observed following treatment of primary mouse epidermal cells with tunicamycin, a specific inhibitor of dolichol-mediated glycosylation. Following 18 h of treatment, the specific binding of [3H]PDBu was reduced to 33-56% of the control value. The total protein synthesis determined by leucine incorporation into acid-insoluble material was not altered by this antibiotic drug. These results suggest that the receptor for phorbol diesters is, or is functionally linked to, a glycoprotein on the cell surface.

AB - A progressive decline in the specific binding of [20-3H]phorbol 12,13-dibutyrate ([3H]PDBu) and glycoprotein synthesis was observed following treatment of primary mouse epidermal cells with tunicamycin, a specific inhibitor of dolichol-mediated glycosylation. Following 18 h of treatment, the specific binding of [3H]PDBu was reduced to 33-56% of the control value. The total protein synthesis determined by leucine incorporation into acid-insoluble material was not altered by this antibiotic drug. These results suggest that the receptor for phorbol diesters is, or is functionally linked to, a glycoprotein on the cell surface.

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JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 3

ER -

Effect of tunicamycin on receptors for tumor promoters

Abstract

ASJC Scopus subject areas

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