Abstract
Purpose: To determine whether trypan blue causes a significant difference in the biomechanical properties (stiffness) of diabetic and nondiabetic anterior lens capsules and to determine whether diabetes significantly alters the stiffness of anterior lens capsules. Setting: Veterans Administration Boston Healthcare Center System, Jamaica Plain, Massachusetts, USA. Methods: In this unmasked prospective randomized controlled experimental study, anterior lens capsules were obtained from diabetic and nondiabetic patients approved for cataract surgery. Four treatment groups were created: (1) capsules of nondiabetic patients without trypan blue; capsules of nondiabetic patients with trypan blue; (3) capsules of diabetic patients without trypan blue; (4) capsules of diabetic patients with trypan blue. Anterior lens capsule stiffness as a function of elastic (Young's) modulus (kilopascals) was measured with the Hysitron TriboIndenter. Results: The use of trypan blue led to significantly stiffer anterior lens capsules (P = .036). Trypan blue had the greatest effect on the stiffness of diabetic lens capsules (P = .046). Conclusion: Trypan blue contributed to increased capsule stiffness during capsulorhexis, especially in diabetic patients.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 318-323 |
| Number of pages | 6 |
| Journal | Journal of Cataract and Refractive Surgery |
| Volume | 35 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2009 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
- Surgery
Cite this
Effect of trypan blue staining on the elastic modulus of anterior lens capsules of diabetic and nondiabetic patients. / Jardeleza, Maria Stephanie R; Daly, Mary K.; Kaufman, Jessica D.; Klapperich, Catherine; Legutko, Paul A.
In: Journal of Cataract and Refractive Surgery, Vol. 35, No. 2, 02.2009, p. 318-323.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of trypan blue staining on the elastic modulus of anterior lens capsules of diabetic and nondiabetic patients
AU - Jardeleza, Maria Stephanie R
AU - Daly, Mary K.
AU - Kaufman, Jessica D.
AU - Klapperich, Catherine
AU - Legutko, Paul A.
PY - 2009/2
Y1 - 2009/2
N2 - Purpose: To determine whether trypan blue causes a significant difference in the biomechanical properties (stiffness) of diabetic and nondiabetic anterior lens capsules and to determine whether diabetes significantly alters the stiffness of anterior lens capsules. Setting: Veterans Administration Boston Healthcare Center System, Jamaica Plain, Massachusetts, USA. Methods: In this unmasked prospective randomized controlled experimental study, anterior lens capsules were obtained from diabetic and nondiabetic patients approved for cataract surgery. Four treatment groups were created: (1) capsules of nondiabetic patients without trypan blue; capsules of nondiabetic patients with trypan blue; (3) capsules of diabetic patients without trypan blue; (4) capsules of diabetic patients with trypan blue. Anterior lens capsule stiffness as a function of elastic (Young's) modulus (kilopascals) was measured with the Hysitron TriboIndenter. Results: The use of trypan blue led to significantly stiffer anterior lens capsules (P = .036). Trypan blue had the greatest effect on the stiffness of diabetic lens capsules (P = .046). Conclusion: Trypan blue contributed to increased capsule stiffness during capsulorhexis, especially in diabetic patients.
AB - Purpose: To determine whether trypan blue causes a significant difference in the biomechanical properties (stiffness) of diabetic and nondiabetic anterior lens capsules and to determine whether diabetes significantly alters the stiffness of anterior lens capsules. Setting: Veterans Administration Boston Healthcare Center System, Jamaica Plain, Massachusetts, USA. Methods: In this unmasked prospective randomized controlled experimental study, anterior lens capsules were obtained from diabetic and nondiabetic patients approved for cataract surgery. Four treatment groups were created: (1) capsules of nondiabetic patients without trypan blue; capsules of nondiabetic patients with trypan blue; (3) capsules of diabetic patients without trypan blue; (4) capsules of diabetic patients with trypan blue. Anterior lens capsule stiffness as a function of elastic (Young's) modulus (kilopascals) was measured with the Hysitron TriboIndenter. Results: The use of trypan blue led to significantly stiffer anterior lens capsules (P = .036). Trypan blue had the greatest effect on the stiffness of diabetic lens capsules (P = .046). Conclusion: Trypan blue contributed to increased capsule stiffness during capsulorhexis, especially in diabetic patients.
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UR - http://www.scopus.com/inward/citedby.url?scp=58949097705&partnerID=8YFLogxK
U2 - 10.1016/j.jcrs.2008.10.040
DO - 10.1016/j.jcrs.2008.10.040
M3 - Article
C2 - 19185249
AN - SCOPUS:58949097705
VL - 35
SP - 318
EP - 323
JO - Journal of Cataract and Refractive Surgery
JF - Journal of Cataract and Refractive Surgery
SN - 0886-3350
IS - 2
ER -