Effect of trypan blue staining on the elastic modulus of anterior lens capsules of diabetic and nondiabetic patients

Maria Stephanie R Jardeleza, Mary K. Daly, Jessica D. Kaufman, Catherine Klapperich, Paul A. Legutko

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Purpose: To determine whether trypan blue causes a significant difference in the biomechanical properties (stiffness) of diabetic and nondiabetic anterior lens capsules and to determine whether diabetes significantly alters the stiffness of anterior lens capsules. Setting: Veterans Administration Boston Healthcare Center System, Jamaica Plain, Massachusetts, USA. Methods: In this unmasked prospective randomized controlled experimental study, anterior lens capsules were obtained from diabetic and nondiabetic patients approved for cataract surgery. Four treatment groups were created: (1) capsules of nondiabetic patients without trypan blue; capsules of nondiabetic patients with trypan blue; (3) capsules of diabetic patients without trypan blue; (4) capsules of diabetic patients with trypan blue. Anterior lens capsule stiffness as a function of elastic (Young's) modulus (kilopascals) was measured with the Hysitron TriboIndenter. Results: The use of trypan blue led to significantly stiffer anterior lens capsules (P = .036). Trypan blue had the greatest effect on the stiffness of diabetic lens capsules (P = .046). Conclusion: Trypan blue contributed to increased capsule stiffness during capsulorhexis, especially in diabetic patients.

Original languageEnglish (US)
Pages (from-to)318-323
Number of pages6
JournalJournal of Cataract and Refractive Surgery
Volume35
Issue number2
DOIs
StatePublished - Feb 2009
Externally publishedYes

Fingerprint

Anterior Capsule of the Lens
Trypan Blue
Elastic Modulus
Staining and Labeling
Capsules
Capsulorhexis
Jamaica
United States Department of Veterans Affairs
Cataract
Lenses
Delivery of Health Care

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Surgery

Cite this

Effect of trypan blue staining on the elastic modulus of anterior lens capsules of diabetic and nondiabetic patients. / Jardeleza, Maria Stephanie R; Daly, Mary K.; Kaufman, Jessica D.; Klapperich, Catherine; Legutko, Paul A.

In: Journal of Cataract and Refractive Surgery, Vol. 35, No. 2, 02.2009, p. 318-323.

Research output: Contribution to journalArticle

Jardeleza, Maria Stephanie R ; Daly, Mary K. ; Kaufman, Jessica D. ; Klapperich, Catherine ; Legutko, Paul A. / Effect of trypan blue staining on the elastic modulus of anterior lens capsules of diabetic and nondiabetic patients. In: Journal of Cataract and Refractive Surgery. 2009 ; Vol. 35, No. 2. pp. 318-323.
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