Abstract
1. 1.|The effect of chemical modification of membrane proteins on the heat response of erythrocytes has been studied. 2. 2.|Erythrocytes preincubated with N-ethylmaleimide (NEM) or p-chloromercuribenzoic acid (pCMB), followed by heating at temperatures above 44°C, indicated a decreased thermal stability. 3. 3.|The action of pCMB is much more toxic to erythrocytes than the effect of NEM. 4. 4.|These results suggest that modification of proteins by SH reagents could be responsible for changes in the structural integrity of pig erythrocyte plasma membrane leading to their reduced thermal stability.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 141-147 |
| Number of pages | 7 |
| Journal | Journal of Thermal Biology |
| Volume | 19 |
| Issue number | 2 |
| DOIs | |
| State | Published - Apr 1994 |
| Externally published | Yes |
Keywords
- Erythrocytes
- SH reagents
- heat
- membrane proteins
- thermal stability
ASJC Scopus subject areas
- Biochemistry
- Physiology
- Agricultural and Biological Sciences(all)
- Developmental Biology
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Cite this
Jóźwiak, Z., Gaczynska, M., & Soszynski, M. (1994). Effect of thiol modification on the thermal stability of erythrocyte membranes. Journal of Thermal Biology, 19(2), 141-147. https://doi.org/10.1016/0306-4565(94)90062-0