Effect of the co‐carcinogen benzo[e] pyrene on microsome‐mediated chemical mutagenesis in salmonella typhimurium

T. Kameswar Rao; J. A. Young; C. E. Weeks; T. J. Slaga; J. L. Epler

doi:10.1002/em.2860010203

Effect of the co‐carcinogen benzo[e] pyrene on microsome‐mediated chemical mutagenesis in salmonella typhimurium

T. Kameswar Rao, J. A. Young, C. E. Weeks, T. J. Slaga, J. L. Epler

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Chemical agents that possess the ability to alter tumorigenicity of carcinogens (administered at subthreshold dose) constitute a major health hazard. We have employed the Ames Salmonella assay to examine the effect of co‐carcinogenic benzo[e] pyrene (B[e]P) on microsome‐mediated chemical mutagenesis. B[e]P enhanced the mutagenic activity induced by 2‐acetylaminofluorene (2‐AAF) and benzo[a] pyrene (B[a]P) in strains TA1538 and TA98. Enhancement was also noted with N‐hydroxy‐2‐AAF (the proximal metabolite of 2‐AAF) but not with an ultimate carcinogenic form (N‐acetoxy‐2‐AAF). These results suggest the use of this approach to detect chemical agents that possess the ability to alter the activity of mutagenic or carcinogenic chemicals.

Original language	English (US)
Pages (from-to)	105-112
Number of pages	8
Journal	Environmental Mutagenesis
Volume	1
Issue number	2
DOIs	https://doi.org/10.1002/em.2860010203
State	Published - 1979
Externally published	Yes

Keywords

Ames assay
co‐carcinogen
co‐mutagen
tumor promoter

ASJC Scopus subject areas

Genetics
Health, Toxicology and Mutagenesis

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10.1002/em.2860010203

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title = "Effect of the co‐carcinogen benzo[e] pyrene on microsome‐mediated chemical mutagenesis in salmonella typhimurium",

abstract = "Chemical agents that possess the ability to alter tumorigenicity of carcinogens (administered at subthreshold dose) constitute a major health hazard. We have employed the Ames Salmonella assay to examine the effect of co‐carcinogenic benzo[e] pyrene (B[e]P) on microsome‐mediated chemical mutagenesis. B[e]P enhanced the mutagenic activity induced by 2‐acetylaminofluorene (2‐AAF) and benzo[a] pyrene (B[a]P) in strains TA1538 and TA98. Enhancement was also noted with N‐hydroxy‐2‐AAF (the proximal metabolite of 2‐AAF) but not with an ultimate carcinogenic form (N‐acetoxy‐2‐AAF). These results suggest the use of this approach to detect chemical agents that possess the ability to alter the activity of mutagenic or carcinogenic chemicals.",

keywords = "Ames assay, co‐carcinogen, co‐mutagen, tumor promoter",

author = "{Kameswar Rao}, T. and Young, {J. A.} and Weeks, {C. E.} and Slaga, {T. J.} and Epler, {J. L.}",

year = "1979",

doi = "10.1002/em.2860010203",

language = "English (US)",

volume = "1",

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AU - Kameswar Rao, T.

AU - Young, J. A.

AU - Weeks, C. E.

AU - Slaga, T. J.

AU - Epler, J. L.

PY - 1979

Y1 - 1979

N2 - Chemical agents that possess the ability to alter tumorigenicity of carcinogens (administered at subthreshold dose) constitute a major health hazard. We have employed the Ames Salmonella assay to examine the effect of co‐carcinogenic benzo[e] pyrene (B[e]P) on microsome‐mediated chemical mutagenesis. B[e]P enhanced the mutagenic activity induced by 2‐acetylaminofluorene (2‐AAF) and benzo[a] pyrene (B[a]P) in strains TA1538 and TA98. Enhancement was also noted with N‐hydroxy‐2‐AAF (the proximal metabolite of 2‐AAF) but not with an ultimate carcinogenic form (N‐acetoxy‐2‐AAF). These results suggest the use of this approach to detect chemical agents that possess the ability to alter the activity of mutagenic or carcinogenic chemicals.

AB - Chemical agents that possess the ability to alter tumorigenicity of carcinogens (administered at subthreshold dose) constitute a major health hazard. We have employed the Ames Salmonella assay to examine the effect of co‐carcinogenic benzo[e] pyrene (B[e]P) on microsome‐mediated chemical mutagenesis. B[e]P enhanced the mutagenic activity induced by 2‐acetylaminofluorene (2‐AAF) and benzo[a] pyrene (B[a]P) in strains TA1538 and TA98. Enhancement was also noted with N‐hydroxy‐2‐AAF (the proximal metabolite of 2‐AAF) but not with an ultimate carcinogenic form (N‐acetoxy‐2‐AAF). These results suggest the use of this approach to detect chemical agents that possess the ability to alter the activity of mutagenic or carcinogenic chemicals.

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KW - co‐mutagen

KW - tumor promoter

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Effect of the co‐carcinogen benzo[e] pyrene on microsome‐mediated chemical mutagenesis in salmonella typhimurium

Abstract

Keywords

ASJC Scopus subject areas

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