Effect of the antitumor drug vinblastine on nuclear βII-tubulin in cultured rat kidney mesangial cells

Consuelo Walss-Bass, Jeffrey I. Kreisberg, Richard F. Ludueña

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Tubulin, the main component of microtubules, is a major target for antitumor drugs such as vinblastine. We have recently discovered that the βII isotype of tubulin is present in the nuclei of cultured rat kidney mesangial cells, smooth-muscle-like cells present in the renal glomerular mesangium (Walss C, Kreisberg JI, Ludueña RF: Cell Motil Cytoskeleton 42: 274-284, 1999). Here, we have investigated the effect of vinblastine on nuclear βII-tubulin in these cells. We have found that, at concentrations of 15 nM and higher, vinblastine caused a reversible loss of βII-tubulin from the nucleus. Our results raise the possibility that nuclear βII-tubulin constitutes a population of tubulin that could be a novel target for antitumor drugs such as vinblastine.

Original languageEnglish (US)
Pages (from-to)15-20
Number of pages6
JournalInvestigational New Drugs
Volume21
Issue number1
DOIs
StatePublished - Feb 2003

Fingerprint

Mesangial Cells
Vinblastine
Tubulin
Antineoplastic Agents
Glomerular Mesangium
Cytoskeleton
Microtubules
Smooth Muscle Myocytes
Kidney
Population

Keywords

  • DNA fragmentation
  • Microtubules
  • Nuclear localization
  • Tubulin isotypes
  • Vinblastine

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Effect of the antitumor drug vinblastine on nuclear βII-tubulin in cultured rat kidney mesangial cells. / Walss-Bass, Consuelo; Kreisberg, Jeffrey I.; Ludueña, Richard F.

In: Investigational New Drugs, Vol. 21, No. 1, 02.2003, p. 15-20.

Research output: Contribution to journalArticle

Walss-Bass, Consuelo ; Kreisberg, Jeffrey I. ; Ludueña, Richard F. / Effect of the antitumor drug vinblastine on nuclear βII-tubulin in cultured rat kidney mesangial cells. In: Investigational New Drugs. 2003 ; Vol. 21, No. 1. pp. 15-20.
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