Effect of the antitumor drug vinblastine on nuclear βII-tubulin in cultured rat kidney mesangial cells

Consuelo Walss-Bass, Jeffrey I. Kreisberg, Richard F. Ludueña

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Tubulin, the main component of microtubules, is a major target for antitumor drugs such as vinblastine. We have recently discovered that the βII isotype of tubulin is present in the nuclei of cultured rat kidney mesangial cells, smooth-muscle-like cells present in the renal glomerular mesangium (Walss C, Kreisberg JI, Ludueña RF: Cell Motil Cytoskeleton 42: 274-284, 1999). Here, we have investigated the effect of vinblastine on nuclear βII-tubulin in these cells. We have found that, at concentrations of 15 nM and higher, vinblastine caused a reversible loss of βII-tubulin from the nucleus. Our results raise the possibility that nuclear βII-tubulin constitutes a population of tubulin that could be a novel target for antitumor drugs such as vinblastine.

Original languageEnglish (US)
Pages (from-to)15-20
Number of pages6
JournalInvestigational New Drugs
Volume21
Issue number1
DOIs
StatePublished - Feb 1 2003

Keywords

  • DNA fragmentation
  • Microtubules
  • Nuclear localization
  • Tubulin isotypes
  • Vinblastine

ASJC Scopus subject areas

  • Oncology
  • Pharmacology
  • Pharmacology (medical)

Fingerprint Dive into the research topics of 'Effect of the antitumor drug vinblastine on nuclear β<sub>II</sub>-tubulin in cultured rat kidney mesangial cells'. Together they form a unique fingerprint.

Cite this