Effect of Sulfo Lewis C on smoke inhalation injury in an ovine model

Osamu Tasaki, David W. Mozingo, Satoshi Ishihara, William W. Brinkley, Avery A. Johnson, Richard H. Smith, Om Srivastava, Arthur D. Mason, Basil A. Pruitt, William G. Cioffi

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Objective: To evaluate the effect of Sulfo Lewis C (SO3- 3aGal13GlcNAc-O(CH2)8-COOMe), a putative ligand of selectins, on smoke inhalation injury. Design: Prospective animal study with concurrent controls. Setting: An animal laboratory. Subjects: Twelve 1-yr-old female sheep, weighing 24 to 33 kg. Interventions: Twelve sheep received nine exposure units of smoke generated bY thermolysis of pine woodchips (80 g). Group 1 (n = 6) was untreated. Group 2 (n = 6) was treated with an intravenous infusion of Sulfo Lewis C after smoke exposure. Animals were killed 48 hrs after injury. Measurements and Main Results: Cardiopulmonary variables and blood gases were measured serially. Granulocyte free-radical production was measured before smoke exposure and at 4 and 48 hrs after injury. Ventilation/perfusion distribution (V̇(A)/Q̇) was analyzed using the multiple inert gas elimination technique. Granulocyte free-radical production was increased after smoke exposure in both groups. Oxygenation was significantly improved by the administration of Sulfo Lewis C. V̇(A)/Q̇. analysis demonstrated significantly less blood flow to low V̇A/Q lung segments in treated animals. Conclusions: Selectin blockade attenuated lung injury after smoke exposure. These data support the hypothesis that neutrophils play a pivotal role in smoke inhalation injury.

Original languageEnglish (US)
Pages (from-to)1238-1243
Number of pages6
JournalCritical care medicine
Volume26
Issue number7
DOIs
StatePublished - 1998

Keywords

  • Adhesion molecule
  • Free radical
  • Granulocyte
  • Hypoxemia
  • Lung injury
  • Multiple inert gas elimination technique
  • Ovine
  • Selectin
  • Smoke inhalation
  • Sulfo Lewis C

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

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