Effect of radiofrequency radiation on mRNA expression in cultured rodent cells.

J. E. Parker, J. L. Kiel, W. D. Winters

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Four rodent cell lines were exposed to 2450 MHz microwave radiation at a Specific Absorption Rate (SAR) of 103.5 +/- 4.2 W/kg for varying lengths of time at 37 degrees, 40 degrees, 42 degrees and 45 degrees C. mRNA was extracted from microwave-exposed and sham-exposed cells and dot blotted or Northern blotted to nitrocellulose. Radioisotope labelled DNA probes of oncogenes, heat shock protein or long terminal repeat sequences were hybridized to the mRNA, and the resulting autoradiographs analyzed for differences in levels of mRNA expression between exposed and nonexposed samples. With the cell lines and probes used in this study no significant differences in mRNA expression were observed after microwave exposure.

Original languageEnglish (US)
Pages (from-to)129-134
Number of pages6
JournalPhysiological Chemistry and Physics and Medical NMR
Volume20
Issue number2
StatePublished - 1988
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Physiology
  • Spectroscopy

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