Effect of proteolysis on the electron spin resonance spectra of maleimide spin labeled erythrocyte membrane

Grzegorz Bartosz, Maria Gaczyńska

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The ratio of low-field amplitudes of weakly and strongly immobilized signals of ESR spectra of a maleimide spin label bound to erythrocyte membranes (hw/hs) increases progressively during incubation at 37δC. This increase is due to the 'self-digestion' of membrane proteins by endogenous proteinases and is attenuated by proteinase inhibitors. Digestion of membranes with chymotrypsin also increases the hw/hs, ratio. These results suggest a need for a careful interpretation of data from spin-labeled membrane proteins, especially in experiments involving prolonged incubations of membrane preparations when the proteolytic effects may be significant.

Original languageEnglish (US)
Pages (from-to)175-178
Number of pages4
JournalBBA - Biomembranes
Volume821
Issue number2
DOIs
StatePublished - Dec 5 1985
Externally publishedYes

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Keywords

  • ESR
  • Erythrocyte membrane
  • Maleimide spin label
  • Proteolysis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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