Effect of non-lytic concentrations of Brij series detergents on the metabolism-independent ion permeability properties of human erythrocytes

A. Miseta, P. Bogner, A. Szarka, M. Kellermayer, C. Galambos, D. N. Wheatley, I. L. Cameron

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Subcritical micellar concentrations (sub-CMC) of Brij-series detergents alter ion movements between human erythrocytes and their environment when metabolism has been slowed down by incubation at zero degrees centigrade. The effect of nonhemolytic concentrations of detergents on the erythrocyte K+ and Na+ movements is described. Results indicate a significant difference in monovalent cation movements, depending on the number of hydrophilic polyoxyethylene units (n). There is an increasing loss of K+ and gain of Na+ as n increases from 4 to 20. Where n > or = 21, ion movements are not significantly different from those found in erythrocytes not exposed to detergents. The carbon chain length of the detergent fatty acid residue (10–18 carbons) appears to be relatively unimportant, but detergents with unsaturated (oleic acid) hydrophobic regions potentiate K+ release and Na+ uptake when compared to the corresponding saturated fatty acid (stearic acid). The erythrocyte stabilizing effect of detergents against hypo-osmotic shock correlates well with the increase of monovalent ion traffic and the mobility of membrane lipids revealed by fluorescence anisotropy measurements.

Original languageEnglish (US)
Pages (from-to)2563-2568
Number of pages6
JournalBiophysical Journal
Volume69
Issue number6
DOIs
StatePublished - 1995
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics

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