TY - JOUR
T1 - Effect of nanoemulsion on dental unit waterline biofilm
AU - Ramalingam, Karthikeyan
AU - Frohlich, Nicholas C.
AU - Lee, Valerie A.
N1 - Funding Information:
This study was supported by NIH/NIDCR K08DE018003 .
PY - 2013/9
Y1 - 2013/9
N2 - Bacterial biofilm in dental unit waterlines (DUWLs) is a widespread problem and poses a potentially significant risk of infection to dental staff and patients. The present study investigates the level and composition of bacterial contamination of dental chair syringe waterlines and investigates the efficacy of a cetylpyridinium chloride-containing nanoemulsion disinfectant in reducing bacterial loads. Waterline biofilms exposed to nanoemulsion for 1 hour, 6 hours, 12 hours, 24 hours, 48 hours, and 72 hours showed high reduction of colonies, and very low counts after 12 hours and 24 hours (67 colony-forming units/mL) were observed. Exposures for 48 hours and 72 hours showed no or few visible colonies (2 colony-forming units/mL). The nanoemulsion employed improves efficacy against microorganisms more than unemulsified components. DNA sequencing showed that the organisms in the waterline biofilm are primarily of soil or water origin. The findings indicate that nanoemulsion effectively disinfects waterlines to consistently meet the American Dental Association (ADA) recommendation.
AB - Bacterial biofilm in dental unit waterlines (DUWLs) is a widespread problem and poses a potentially significant risk of infection to dental staff and patients. The present study investigates the level and composition of bacterial contamination of dental chair syringe waterlines and investigates the efficacy of a cetylpyridinium chloride-containing nanoemulsion disinfectant in reducing bacterial loads. Waterline biofilms exposed to nanoemulsion for 1 hour, 6 hours, 12 hours, 24 hours, 48 hours, and 72 hours showed high reduction of colonies, and very low counts after 12 hours and 24 hours (67 colony-forming units/mL) were observed. Exposures for 48 hours and 72 hours showed no or few visible colonies (2 colony-forming units/mL). The nanoemulsion employed improves efficacy against microorganisms more than unemulsified components. DNA sequencing showed that the organisms in the waterline biofilm are primarily of soil or water origin. The findings indicate that nanoemulsion effectively disinfects waterlines to consistently meet the American Dental Association (ADA) recommendation.
KW - biofilms
KW - dental unit waterlines
KW - disinfection
KW - microorganisms
KW - nanoemulsion
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U2 - 10.1016/j.jds.2013.02.035
DO - 10.1016/j.jds.2013.02.035
M3 - Article
AN - SCOPUS:84885055640
VL - 8
SP - 333
EP - 336
JO - Journal of Dental Sciences
JF - Journal of Dental Sciences
SN - 1991-7902
IS - 3
ER -