Effect of medium composition on results of macrobroth dilution antifungal susceptibility testing of yeasts

G. V. Doern, T. A. Tubert, K. Chapin, M. G. Rinaldi

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

A total of 62 different clinical yeast isolates were examined for susceptibility to four antifungal agents, amphotericin B, 5-fluorocytosine (5-FC) ketoconazole, and miconazole, using synthetic amino acid medium-fungi (SAAM-F), buffered yeast nitrogen broth (BYNB), Kimmig broth, casein-yeast-glucose broth (CYG), antibiotic medium 3-FDA (ANTI-3), and tryptic soy broth (TSB). A macrobroth dilution format was used with MICs determined after incubation from 24 and 48 h. All analyses were performed in duplicate. In general, MICs were more reproducible after 48 h of incubation. Furthermore, with certain medium-antifungal agent combinations, MICs determined after incubation for 48 h were significantly higher than those determined after 24 h. For instance, with 5-FC irrespective of the medium used, >25% of all 48-h MICs were more than one twofold dilution higher than the corresponding MICs determined after incubation for 24 h. Similar observations were made with amphotericin B when tested with BYNB and CYG and with the imidazoles when tested in all of the media except CYG. The actual MICs obtained with the different antifungal agents were clearly influenced by the test medium used. The rank order of amphotericin B MICs according to test medium was as follows: BYNB>SAAM-F=Kimmig=CYG=ANTI-3=TSB. With 5-FC, the following pattern was observed: Kimmig=ANTI-3>SAAM-F=CYG=TSB>BYNB. For both imidazoles, ketoconazole and miconazole, the rank order of MICs according to test medium was BYNB=Kimmig=CYG=ANTI-3=TSB>SAAM-F. The results of this investigation suggested that broth dilution susceptibility testing of yeasts is best performed with an incubation period of 48 h. Furthermore, medium composition can significantly influence the results of such testing.

Original languageEnglish (US)
Pages (from-to)507-511
Number of pages5
JournalJournal of clinical microbiology
Volume24
Issue number4
DOIs
StatePublished - 1986
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)

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