TY - JOUR
T1 - Effect of human TGF-β on the gene expression profile of Schistosoma mansoni adult worms
AU - Oliveira, Katia C.
AU - Carvalho, Mariana L.P.
AU - Verjovski-Almeida, Sergio
AU - Loverde, Philip T.
N1 - Funding Information:
Supported in part by a grant from Fundaçao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) to SVA and by NIH AI 46762 to PTL. KCO and MLPC received postdoctoral and Master fellowships from FAPESP, respectively, and SVA is the recipient of an established investigator fellowship from CNPq. Schistosome material was obtained from the Schistosome Supply Contract (NIAID Contract No. HHSN272201000009I) to Dr. Fred Lewis.
PY - 2012/6
Y1 - 2012/6
N2 - Schistosoma mansoni is responsible for schistosomiasis, a parasitic disease that affects 200 million people worldwide. Molecular mechanisms of host-parasite interaction are complex and involve a crosstalk between host signals and parasite receptors. TGF-β signaling pathway has been shown to play an important role in S. mansoni development and embryogenesis. In particular human (h) TGF-β has been shown to bind to a S. mansoni receptor, transduce a signal that regulates the expression of a schistosome target gene. Here we describe 381 parasite genes whose expression levels are affected by in vitro treatment with hTGF-β. Among these differentially expressed genes we highlight genes related to morphology, development and cell cycle that could be players of cytokine effects on the parasite. We confirm by qPCR the expression changes detected with microarrays for 5 out of 7 selected genes. We also highlight a set of non-coding RNAs transcribed from the same loci of protein-coding genes that are differentially expressed upon hTGF-β treatment. These datasets offer potential targets to be explored in order to understand the molecular mechanisms behind the possible role of hTGF-β effects on parasite biology.
AB - Schistosoma mansoni is responsible for schistosomiasis, a parasitic disease that affects 200 million people worldwide. Molecular mechanisms of host-parasite interaction are complex and involve a crosstalk between host signals and parasite receptors. TGF-β signaling pathway has been shown to play an important role in S. mansoni development and embryogenesis. In particular human (h) TGF-β has been shown to bind to a S. mansoni receptor, transduce a signal that regulates the expression of a schistosome target gene. Here we describe 381 parasite genes whose expression levels are affected by in vitro treatment with hTGF-β. Among these differentially expressed genes we highlight genes related to morphology, development and cell cycle that could be players of cytokine effects on the parasite. We confirm by qPCR the expression changes detected with microarrays for 5 out of 7 selected genes. We also highlight a set of non-coding RNAs transcribed from the same loci of protein-coding genes that are differentially expressed upon hTGF-β treatment. These datasets offer potential targets to be explored in order to understand the molecular mechanisms behind the possible role of hTGF-β effects on parasite biology.
KW - Gene network interactions
KW - Host-parasite cross talk
KW - Microarray analysis
KW - Schistosoma mansoni
KW - TGF-β signaling
KW - ncRNAs
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U2 - 10.1016/j.molbiopara.2012.02.008
DO - 10.1016/j.molbiopara.2012.02.008
M3 - Article
C2 - 22387759
AN - SCOPUS:84859624202
SN - 0166-6851
VL - 183
SP - 132
EP - 139
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 2
ER -