Effect of gestational age, corticosteroids, and birth on expression of prostanoid EP receptor genes in lamb and baboon ductus arteriosus

Gordon C S Smith, Wen X. Wu, Mark J Nijland, Stephen V. Koenen, Peter W. Nathanielsz

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The aim of this study was to determine the effect of corticosteroids, gestational age, and birth on the expression of genes encoding prostanoid receptors in the lamb and baboon ductus arteriosus. The ductus arteriosus was obtained from 34 lambs and eight baboons, including chronically instrumented fetuses of both species exposed to either corticosteroid or vehicle. Expression of prostanoid receptor genes was quantified using Northern blot analysis relative to each of two housekeeping genes. Expression of both the EP3 and EP4, receptor genes was detected in lamb ductus and the level of expression of both genes was unaffected by corticosteroids. Expression of the EP4 receptor gene was lower in the ductus obtained from term lambs compared with preterm lambs and was lower still in neonatal animals, whereas no variation was observed in EP3 receptor gene expression. Expression of the EP4 receptor gene was also confirmed in fetal baboon ductus arteriosus, and maternal administration of corticosteroid did not reduce EP4 receptor gene expression in the baboon. We conclude that advancing gestational age and birth may inhibit prostaglandin E2-mediated relaxation of the ductus through a corticosteroid-independent reduction in EP4 receptor gene expression.

Original languageEnglish (US)
Pages (from-to)697-704
Number of pages8
JournalJournal of Cardiovascular Pharmacology
Volume37
Issue number6
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Ductus Arteriosus
Papio
Gestational Age
Prostaglandins
Adrenal Cortex Hormones
Parturition
Gene Expression
Genes
Newborn Animals
Essential Genes
Dinoprostone
Northern Blotting
Fetus
Mothers

Keywords

  • Ductus arteriosus
  • Fetus
  • Genetics
  • Neonate
  • Prostaglandin
  • Receptors

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

Cite this

Effect of gestational age, corticosteroids, and birth on expression of prostanoid EP receptor genes in lamb and baboon ductus arteriosus. / Smith, Gordon C S; Wu, Wen X.; Nijland, Mark J; Koenen, Stephen V.; Nathanielsz, Peter W.

In: Journal of Cardiovascular Pharmacology, Vol. 37, No. 6, 2001, p. 697-704.

Research output: Contribution to journalArticle

@article{560d5e9b0c3e480f8b6367308e1918bf,
title = "Effect of gestational age, corticosteroids, and birth on expression of prostanoid EP receptor genes in lamb and baboon ductus arteriosus",
abstract = "The aim of this study was to determine the effect of corticosteroids, gestational age, and birth on the expression of genes encoding prostanoid receptors in the lamb and baboon ductus arteriosus. The ductus arteriosus was obtained from 34 lambs and eight baboons, including chronically instrumented fetuses of both species exposed to either corticosteroid or vehicle. Expression of prostanoid receptor genes was quantified using Northern blot analysis relative to each of two housekeeping genes. Expression of both the EP3 and EP4, receptor genes was detected in lamb ductus and the level of expression of both genes was unaffected by corticosteroids. Expression of the EP4 receptor gene was lower in the ductus obtained from term lambs compared with preterm lambs and was lower still in neonatal animals, whereas no variation was observed in EP3 receptor gene expression. Expression of the EP4 receptor gene was also confirmed in fetal baboon ductus arteriosus, and maternal administration of corticosteroid did not reduce EP4 receptor gene expression in the baboon. We conclude that advancing gestational age and birth may inhibit prostaglandin E2-mediated relaxation of the ductus through a corticosteroid-independent reduction in EP4 receptor gene expression.",
keywords = "Ductus arteriosus, Fetus, Genetics, Neonate, Prostaglandin, Receptors",
author = "Smith, {Gordon C S} and Wu, {Wen X.} and Nijland, {Mark J} and Koenen, {Stephen V.} and Nathanielsz, {Peter W.}",
year = "2001",
doi = "10.1097/00005344-200106000-00007",
language = "English (US)",
volume = "37",
pages = "697--704",
journal = "Journal of Cardiovascular Pharmacology",
issn = "0160-2446",
publisher = "Lippincott Williams and Wilkins",
number = "6",

}

TY - JOUR

T1 - Effect of gestational age, corticosteroids, and birth on expression of prostanoid EP receptor genes in lamb and baboon ductus arteriosus

AU - Smith, Gordon C S

AU - Wu, Wen X.

AU - Nijland, Mark J

AU - Koenen, Stephen V.

AU - Nathanielsz, Peter W.

PY - 2001

Y1 - 2001

N2 - The aim of this study was to determine the effect of corticosteroids, gestational age, and birth on the expression of genes encoding prostanoid receptors in the lamb and baboon ductus arteriosus. The ductus arteriosus was obtained from 34 lambs and eight baboons, including chronically instrumented fetuses of both species exposed to either corticosteroid or vehicle. Expression of prostanoid receptor genes was quantified using Northern blot analysis relative to each of two housekeeping genes. Expression of both the EP3 and EP4, receptor genes was detected in lamb ductus and the level of expression of both genes was unaffected by corticosteroids. Expression of the EP4 receptor gene was lower in the ductus obtained from term lambs compared with preterm lambs and was lower still in neonatal animals, whereas no variation was observed in EP3 receptor gene expression. Expression of the EP4 receptor gene was also confirmed in fetal baboon ductus arteriosus, and maternal administration of corticosteroid did not reduce EP4 receptor gene expression in the baboon. We conclude that advancing gestational age and birth may inhibit prostaglandin E2-mediated relaxation of the ductus through a corticosteroid-independent reduction in EP4 receptor gene expression.

AB - The aim of this study was to determine the effect of corticosteroids, gestational age, and birth on the expression of genes encoding prostanoid receptors in the lamb and baboon ductus arteriosus. The ductus arteriosus was obtained from 34 lambs and eight baboons, including chronically instrumented fetuses of both species exposed to either corticosteroid or vehicle. Expression of prostanoid receptor genes was quantified using Northern blot analysis relative to each of two housekeeping genes. Expression of both the EP3 and EP4, receptor genes was detected in lamb ductus and the level of expression of both genes was unaffected by corticosteroids. Expression of the EP4 receptor gene was lower in the ductus obtained from term lambs compared with preterm lambs and was lower still in neonatal animals, whereas no variation was observed in EP3 receptor gene expression. Expression of the EP4 receptor gene was also confirmed in fetal baboon ductus arteriosus, and maternal administration of corticosteroid did not reduce EP4 receptor gene expression in the baboon. We conclude that advancing gestational age and birth may inhibit prostaglandin E2-mediated relaxation of the ductus through a corticosteroid-independent reduction in EP4 receptor gene expression.

KW - Ductus arteriosus

KW - Fetus

KW - Genetics

KW - Neonate

KW - Prostaglandin

KW - Receptors

UR - http://www.scopus.com/inward/record.url?scp=0035009096&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035009096&partnerID=8YFLogxK

U2 - 10.1097/00005344-200106000-00007

DO - 10.1097/00005344-200106000-00007

M3 - Article

C2 - 11392466

AN - SCOPUS:0035009096

VL - 37

SP - 697

EP - 704

JO - Journal of Cardiovascular Pharmacology

JF - Journal of Cardiovascular Pharmacology

SN - 0160-2446

IS - 6

ER -