Effect of exercise intensity on skeletal muscle malonyl-CoA and acetyl- CoA carboxylase

B. B. Rasmussen, W. W. Winder

Research output: Contribution to journalArticlepeer-review

131 Scopus citations


Malonyl-CoA is synthesized by acetyl-CoA carboxylase (ACC) and is an inhibitor of fatty acid oxidation. Exercise induces a decline in skeletal muscle malonyl-CoA, which is accompanied by inactivation of ACC and increased activity of AMP-activated protein kinase (AMPK). This study was designed to determine the effect of exercise intensity on the enzyme kinetics of ACC, malonyl-CoA levels, and AMPK activity in skeletal muscle. Male Sprague- Dawley rats were killed (pentobarbital sodium anesthesia) at rest or after 5 min of exercise (10, 20, 30, or 40 m/min at 5% grade). The fast-twitch red and white regions of the quadriceps muscle were excised and frozen in liquid nitrogen. A progressive decrease in red quadriceps ACC maximal velocity (from 28.6 ± 1.5 to 14.3 ± 0.7 nmol · g-1 · min-1, P < 0.05), an increase in activation constant for citrate, and a decrease in malonyl-CoA (from 1.9 ± 0.2 to 0.9 ± 0.1 nmol/g, P < 0.05) were seen with the increase in exercise intensity from rest to 40 m/min. AMPK activity increased more than twofold. White quadriceps ACC activity decreased only during intense exercise. We conclude that the extent of ACC inactivation during short-term exercise is dependent on exercise intensity.

Original languageEnglish (US)
Pages (from-to)1104-1109
Number of pages6
JournalJournal of applied physiology
Issue number4
StatePublished - Oct 1997
Externally publishedYes


  • Adenosine 5'-monophosphate-activated protein
  • Coenzyme A
  • Fatty acid oxidation
  • Quadriceps

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)


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