Effect of ethanol on intestinal (Na, K) ATPase and intestinal thiamine transport in rats

A. M. Hoyumpa, S. G. Nichols, F. A. Wilson, S. Schenker

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50 Scopus citations


The authors assessed the effect of ethanol on this enzyme: (Na, K) ATPase was assayed in the brush-border membrane and BLM fractions of rat intestinal mucosal cells. In the jejunum, BLM (Na, K) ATPase activity was 13-fold greater than in the corresponding brush-border preparation. Ethanol, 0.5M, added in vitro to the BLM preparation, reduced (Na, K) ATPase activity in the jejunum to 41% (p< 0.001) of control. Ethanol 0.1M, 0.2M, 0.3M, 0.5M, 0.7M, and 1.0M in vitro decreased jejunal BLM (Na, K) ATpase activities to 94%, 78%, 58%, 41%, 28%, and 17% of control value (for all but 94%, p < 0.001), respectively. In addition, 1 hr after oral administration of ethanol, 50, 100, 250, 500, and 750 mg/100 gm body weight, ATPase activity declined to 73%, 52%, 52%, 62%, and 48% of control, respectively (for all, p < 0.01). The same doses of ethanol decreased the serosal appearance rate of 0.5 μM thiamine to 82%, 48%, 50%, 51%, and 51% of control, respectively (for all but 82%, p < 0.05). These data clearly show an association between ethanol inhibition of BLM (Na, K) ATPase and decreased thiamine transport, but whether this association is one of cause and effect requires further study.

Original languageEnglish (US)
Pages (from-to)1086-1095
Number of pages10
JournalJournal of Laboratory and Clinical Medicine
Issue number6
StatePublished - 1977
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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