Effect of endothelin blockade on pressure natriuresis in nitric oxide-deficient hypertensive rats

Lourdes A. Fortepiani, Jean Jacques Janvier, M. Clara Ortíz, Noemí M. Atucha, Joaquín García-Estañ

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Objective. Chronic inhibition of nitric oxide synthesis has been shown to cause arterial hypertension and an important blunting of the pressure diuresis and natriuresis response. The mechanisms mediating these abnormalities are not completely established. We therefore studied the effects of endothelin on these alterations. Materials and methods. Pressure diuretic and natriuretic relationships were evaluated in rats treated chronically (3 weeks) with the nitric oxide synthesis inhibitor N(ω)-nitro-L-arginine methyl ester (L-NAME; 40 mg/kg per day), alone or in combination with bosentan sodium salt (acute treatment: 10 mg/kg, intravenously; chronic treatment: 10 mg/kg per day). Results. Chronic treatment with L-NAME significantly elevated mean arterial pressure (143.7 ± 2.8 mmHg versus 102.8 ± 1.6 in controls), reduced the glomerular filtration rate and renal blood flow and shifted the pressure diuretic and natriuretic responses to the right. Treatment with bosentan, either acute or chronically, did not attenuate the arterial hypertension of the L-NAME-treated rats but normalized the glomerular filtration rate and renal blood flow. In spite of the normalization of renal hemodynamics, the pressure diuretic and natriuretic responses of the bosentan-treated groups were not normalized, although chronic bosentan significantly improved the pressure natriuretic response. Conclusions. These results indicate that endothelin participates in the renal hemodynamic and excretory alterations that follow chronic inhibition of nitric oxide synthesis. However, the arterial hypertension is not mediated by endothelin activation.

Original languageEnglish (US)
Pages (from-to)287-291
Number of pages5
JournalJournal of Hypertension
Volume17
Issue number2
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

Keywords

  • Arterial hypertension
  • Kidney
  • Renal hemodynamics
  • Renal sodium handling

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine

Fingerprint Dive into the research topics of 'Effect of endothelin blockade on pressure natriuresis in nitric oxide-deficient hypertensive rats'. Together they form a unique fingerprint.

  • Cite this