Effect of enamel microabrasion on bacterial colonization

Adriana Segura, Kevin J. Donly, James S. Wefel, David Drake

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Purpose: To determine if microabraded surfaces inhibit Streptococcus mutans colonization. Materials and Methods: Ten extracted molars were obtained and sectioned in half mesio-distally. The mesial side was treated with microabrasion (PREMA Compound). The distal surface was designated as the control site. A plastic tube was secured on each surface with resin composite, exposing a surface of 13.2 mm2. S. mutans 10499 was cultured in TSB-YE supplemented with 5% sucrose and 2.5% glucose was added to each secured site in addition to 50 μl of cell suspension. Samples were incubated in a 5% CO2 chamber for 7 days at 37°C. Samples were taken from each site, incubated with dextranase (75 μg 1ml), sonicated, and vortexed to disrupt cellular aggregates as much as possible. Samples were then plated on Mitis-Salivarius agar using a spiral plating system. Enumeration of numbers of colonizing bacteria was achieved following standard spiral plating system methodology. Results: The mean for the microabraded group was 1.12 × 102 cfu/tooth and for the control it was 1.15 ± 104. Analyzine the data revealed a significant difference (P= 0.0188).

Original languageEnglish (US)
Pages (from-to)272-274
Number of pages3
JournalAmerican journal of dentistry
Volume10
Issue number6
StatePublished - Dec 1 1997
Externally publishedYes

ASJC Scopus subject areas

  • Dentistry(all)

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