Effect of enamel microabrasion on bacterial colonization

Adriana Segura, Kevin J Donly, James S. Wefel, David Drake

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Purpose: To determine if microabraded surfaces inhibit Streptococcus mutans colonization. Materials and Methods: Ten extracted molars were obtained and sectioned in half mesio-distally. The mesial side was treated with microabrasion (PREMA Compound). The distal surface was designated as the control site. A plastic tube was secured on each surface with resin composite, exposing a surface of 13.2 mm2. S. mutans 10499 was cultured in TSB-YE supplemented with 5% sucrose and 2.5% glucose was added to each secured site in addition to 50 μl of cell suspension. Samples were incubated in a 5% CO2 chamber for 7 days at 37°C. Samples were taken from each site, incubated with dextranase (75 μg 1ml), sonicated, and vortexed to disrupt cellular aggregates as much as possible. Samples were then plated on Mitis-Salivarius agar using a spiral plating system. Enumeration of numbers of colonizing bacteria was achieved following standard spiral plating system methodology. Results: The mean for the microabraded group was 1.12 × 102 cfu/tooth and for the control it was 1.15 ± 104. Analyzine the data revealed a significant difference (P= 0.0188).

Original languageEnglish (US)
Pages (from-to)272-274
Number of pages3
JournalAmerican Journal of Dentistry
Volume10
Issue number6
StatePublished - 1997
Externally publishedYes

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Enamel Microabrasion
Streptococcus mutans
Dextranase
Composite Resins
Plastics
Agar
Sucrose
Suspensions
Tooth
Bacteria
Glucose

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Effect of enamel microabrasion on bacterial colonization. / Segura, Adriana; Donly, Kevin J; Wefel, James S.; Drake, David.

In: American Journal of Dentistry, Vol. 10, No. 6, 1997, p. 272-274.

Research output: Contribution to journalArticle

Segura, Adriana ; Donly, Kevin J ; Wefel, James S. ; Drake, David. / Effect of enamel microabrasion on bacterial colonization. In: American Journal of Dentistry. 1997 ; Vol. 10, No. 6. pp. 272-274.
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