Effect of dietary restriction on liver protein synthesis in rats

M. C. Birchenall-Sparks, M. S. Roberts, J. Staecker, J. P. Hardwick, A. Richardson

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38 Scopus citations

Abstract

At 6 wk of age, male Fischer F344 rats were fed a purified, casein-based diet either ad libitum or in the amount of 60% of the diet consumed by the rats fed ad libitum (restricted diet). Hepatocytes were isolated from the rats between 2.5 and 19 mo of age. The protein content of the hepatocytes isolated from the rats fed the restricted amount of diet was significantly lower than that of hepatocytes isolated from rats fed ad libitum. The DNA and RNA content of the hepatocytes were similar for the rats fed the two dietary regimens. The absolute rate of protein synthesis for hepatocytes isolated from rats fed ad libitum decreased 55% between 2.5 and 19 mo of age. However, the rate of protein synthesis by hepatocytes from rats fed the restricted amount of diet decreased only slightly with increasing age. At 19 mo of age, the rate of protein synthesis by hepatocytes from the rats fed the restricted amount of diet was significantly higher than the rate of protein synthesis for hepatocytes from rats fed ad libitum. Therefore, dietary restriction retards the age-related decline in liver protein synthesis.

Original languageEnglish (US)
Pages (from-to)944-950
Number of pages7
JournalJournal of Nutrition
Volume115
Issue number7
DOIs
StatePublished - Jan 1 1985

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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    Birchenall-Sparks, M. C., Roberts, M. S., Staecker, J., Hardwick, J. P., & Richardson, A. (1985). Effect of dietary restriction on liver protein synthesis in rats. Journal of Nutrition, 115(7), 944-950. https://doi.org/10.1093/jn/115.7.944