Effect of dietary restriction on hepatic and renal phosphoenolpyruvate carboxykinase induction in young and old Fischer 344 rats

Holly Van Remmen, Walter F. Ward

Research output: Contribution to journalArticle

3 Scopus citations


Food restriction is known to ameliorate many of the adverse physiologic effects of age. In this study, we have examined the effect of food restriction on the induction of the gluconeogenic enzyme, phosphoenolpyruvate carboxykinase (PEPCK) in liver and kidney following a 12-h fasting period in young (6 month) and old (24 month) ad libitum-fed and food-restricted male Fischer 344 rats. In the liver, following the 12-h fast, the activity of PEPCK increased approximately 2-fold in the young ad libitum fed rats and 3-fold in the young restricted animals. However, PEPCK activity remained unchanged in response to the 12-h fast in the 24 month old ad libitum fed rats. In the old restricted rats, the induction of PEPCK mimicked that of the young rats (PEPCK activity increased 2-fold within the 12-h fasting period). Therefore, dietary restriction not only enhanced the induction response in the liver in young rats, but also restored the induction of hepatic PEPCK in the old animals. In the kidney, there was no effect of age or dietary restriction on the induction of PEPCK as the activity of renal PEPCK did not change in response to the 12-h fast in any of the four groups of rats.

Original languageEnglish (US)
Pages (from-to)263-275
Number of pages13
JournalMechanisms of Ageing and Development
Issue number3
StatePublished - Sep 1 1998



  • Dietary restriction
  • Enzyme induction
  • Fasting
  • Fischer 344 rats
  • Phosphoenolpyruvate carboxykinase
  • Refeeding

ASJC Scopus subject areas

  • Aging
  • Developmental Biology

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