Effect of circulating fibronectin on stimulation of leukocyte oxygen consumption and serum opsonizing function in burned patients

Marek K. Dobke, Georgina Pearson, Cheryl Roberts, Betty Germany, Ellen Heck, Bettie Sue Siler Masters, Charles R. Baxter

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

In a study of 27 thermally burned patients (mean TBSA, 58%; range, 32-96%) serum fibronectin levels were decreased with parallel decreased oxygen consumption of stimulated peripheral blood phagocytes and decreased EGTA-blocked burn serum opsonizing activity which correlated with serum fibronectin changes postburn. Normal and burn sera fibronectin content also correlated with the opsonizing times for zymosan and Staphylococcus aureus but not for Enterobacteriaceae. Although in vivo 14 cases showed circulating fibronectin 140 μg/ml or lower and a marked decrease in Staphylococcus aureus opsonization, only two patients from this group revealed positive Staphylococcus aureus blood cultures and serum fibronectin levels were higher in patients with Staphylococcus aureus sepsis than in patients with Enterobacteriaceae sepsis. Supplementary experiments on leukocyte oxidative response after zymosan stimulation in normal, fibronectin-depleted, and fibronectin-reconstituted serum demonstrated that the lag period of oxygen burst is a fibronectin-dependent reaction.

Original languageEnglish (US)
Pages (from-to)882-890
Number of pages9
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume23
Issue number10
DOIs
StatePublished - Oct 1983

ASJC Scopus subject areas

  • Surgery
  • Critical Care and Intensive Care Medicine

Fingerprint Dive into the research topics of 'Effect of circulating fibronectin on stimulation of leukocyte oxygen consumption and serum opsonizing function in burned patients'. Together they form a unique fingerprint.

  • Cite this