Effect of chronic treatment of ethanol on benzodiazepine and picrotoxin sites on the GABA receptor complex in regions of the brain of the rat

S. K. Rastogi, R. Thyagarajan, J. Clothier, M. K. Ticku

Research output: Contribution to journalArticlepeer-review

68 Scopus citations

Abstract

Ethanol has been shown to enhance γ-aminobutyric acid (GABA)ergic transmission. In this study an examination was made of the effect of chronic treatment with ethanol and its withdrawal at 24 h on the binding of [3H]flunitrazepam and [34S]t-butylbicyclophosphorothionate (TBPS) to brain regions in rat. Rats were rendered tolerant to, and dependent on, ethanol by an intragastric intubation method. The affinity (KD) or the binding capacity (Bmax) of [3H]flunitrazepam or [35S]TBPS was not altered by chronic treatment with ethanol or during withdrawal from ethanol. Neither the enhancing effect of GABA on the binding of [3H]glunitrazepain nor its inhibitory effect on the binding of [35TBPS were affected by chronic treatment with ethanol or its withdrawal at 24 h. These results suggest that the sensitivity of benzodiazepine and picrotoxin sites on the oligomeric GABA receptor complex is not affected during tolerance to, or withdrawal from ethanol. It is suggested that the effects of ethanol on GABAergic transmission may be produced at the level of coupled chloride ion channels.

Original languageEnglish (US)
Pages (from-to)1179-1184
Number of pages6
JournalNeuropharmacology
Volume25
Issue number10
DOIs
StatePublished - Oct 1986

Keywords

  • benzodiazepine sites
  • ethanol
  • GABAergic transmission
  • picrotoxin sites
  • tolerance
  • withdrawal

ASJC Scopus subject areas

  • Pharmacology
  • Cellular and Molecular Neuroscience

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