Effect of chronic administration of ethanol on the regulation of the δ-subunit of GABAA receptors in the rat brain

C. R. Marutha Ravindran, Ashok K. Mehta, Maharaj K. Ticku

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

In the present study, we investigated the effect of chronic ethanol (CE) administration on the polypeptide levels of the δ-subunit of GABAA receptors and [3H]muscimol binding to the immunoprecipitated δ-subunit-containing GABAA receptor assemblies in the rat brain. CE administration resulted a down-regulation of polypeptide levels of the δ-subunit of GABAA receptors in the rat cerebellum and hippocampus, whereas there were no changes in the δ-subunit polypeptide levels in the rat cerebral cortex. Further, CE administration caused a down-regulation of native δ-subunit-containing GABAA receptor assemblies in the rat cerebellum as determined by [3H]muscimol binding to the immunoprecipitated receptor assemblies. These results indicate that the δ-subunit-containing GABAA receptors may play a role in chronic ethanol-induced tolerance and dependence.

Original languageEnglish (US)
Pages (from-to)47-52
Number of pages6
JournalBrain Research
Volume1174
Issue number1
DOIs
StatePublished - Oct 12 2007

Keywords

  • δ-Subunit
  • Chronic ethanol
  • GABA receptors
  • Immunoprecipitation
  • Polypeptide levels
  • Radioligand binding

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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